AI Article Synopsis
- Mitochondrial calcium (Ca) is crucial for normal heart function, as its increase boosts ATP production during heart contractions.
- A pathological rise in mitochondrial Ca can cause oxidative stress and disrupt energy production, leading to heart failure symptoms.
- The text outlines a protocol for measuring how mitochondria in guinea pig heart tissue handle calcium.
Article Abstract
Mitochondrial calcium (Ca) plays a key role in regulating normal cardiac function. A physiological increase in mitochondrial matrix calcium [Ca] drives mitochondrial ATP production to meet the high-energy demands during excitation-contraction coupling. However, a pathological increase in [Ca] leads to increased oxidative stress, impaired bioenergetics, and the opening of mitochondrial permeability transition pore (mPTP), a hallmark of the failing heart. Therefore, a better understanding of the [Ca] handling and its role in heart function and dysfunction is of great importance. Here, we describe a detailed protocol for measuring mitochondrial Ca handling in the isolated functionally intact mitochondria from cardiac tissue of the guinea pig.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-2309-1_6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dan-Shen-Yin against ethanol-induced chronic gastric mucosal injury in rats by inhibiting apoptosis via IP3R-controlled calcium release.
J Ethnopharmacol
January 2025
National Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai District, Tianjin 301617, P. R. China; Science and Technology Project of Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China. Electronic address:
Ethnopharmacological Relevance: The pathogenesis of alcoholic gastric injury has long been a significant topic in the medical field. Dan-Shen-Yin (DSY), a classic traditional Chinese medicine formula, has been shown to alleviate gastric mucosal injury and to treat stomachaches in clinical settings. However, the mechanism by which DSY against alcoholic gastric injury requires further investigation.
View Article and Find Full Text PDFCypermethrin triggers oxidative stress, apoptosis, and inflammation in bovine mammary glands by disruption of mitogen-activated protein kinase (MAPK) pathways and calcium homeostasis.
Reprod Toxicol
January 2025
Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Republic of Korea.
Cyano-(3-phenoxyphenyl)methyl]3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropane-1-carboxylate (cypermethrin) is a pyrethroid insecticide that is widely used to repel insects, such as cockroaches and ants. In addition to the target insects, its hazards have been outlined for carp; mice; and the nervous, reproductive, and gastrointestinal systems of humans. However, the effects of cypermethrin on the mammary tissue and milk production in dairy cattle remain unknown.
View Article and Find Full Text PDFMitochondrial DNA alterations in precision oncology: Emerging roles in diagnostics and therapeutics.
Clinics (Sao Paulo)
January 2025
Centro de Investigação Translacional em Oncologia (LIM24), Instituto do Câncer do Estado de São Paulo (ICESP), Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil; Comprehensive Center for Precision Oncology, Universidade de São Paulo, São Paulo, Brazil. Electronic address:
Mitochondria are dynamic organelles essential for vital cellular functions, including ATP production, apoptosis regulation, and calcium homeostasis. Increasing research has highlighted the significance of mitochondrial DNA (mtDNA) content and alterations in the development and progression of various diseases, including cancer. The high mutation rate and vulnerability of mtDNA to damage make these alterations valuable biomarkers for cancer diagnosis, monitoring disease progression, detecting metastasis, and predicting treatment resistance across different tumor types.
View Article and Find Full Text PDFRemodeling of ER Membrane Contact Sites During Cell Division.
Contact (Thousand Oaks)
January 2025
Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Doha, Qatar.
Membrane contact sites (MCS) provide specialized conduits for inter-organelle communications to maintain cellular homeostasis. Most organelles are interconnected, which supports their coordination and function. M-phase (mitosis or meiosis) is associated with dramatic cellular remodeling to support cell division, including the equal distribution of organelles to the two daughter cells.
View Article and Find Full Text PDFMitochondria-Associated Endoplasmic Reticulum Membranes in Microglia: One Contact Site to Rule Them all.
Contact (Thousand Oaks)
January 2025
Department of Biomedicine, Centro de Investigaciones Biológicas Margarita Salas, CSIC, 28040 Madrid, Spain.
Microglia, the resident immune cells of the central nervous system (CNS), play a crucial role in maintaining tissue homeostasis by monitoring and responding to environmental changes through processes such as phagocytosis, cytokine production or synapse remodeling. Their dynamic nature and diverse functions are supported by the regulation of multiple metabolic pathways, enabling microglia to efficiently adapt to fluctuating signals. A key aspect of this regulation occurs at mitochondria-associated ER membranes (MAM), specialized contact sites between the ER and mitochondria.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!