AI Article Synopsis
- * An analysis using LC-MS revealed that picornavirus RNAs mainly contain N-methyladenosine and 2'-O-methylated nucleosides, while iflavirus RNAs showed a wider variety of methylated nucleosides.
- * Furthermore, the study found tRNA molecules with specific methylation patterns in the iflavirus RNAs, indicating they are packed selectively and are typically associated with other viruses.
Article Abstract
The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles; thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC-MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N -methyladenosine and 2'-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m A) and 5-methylcytidine (m C). Mapping of m A and m C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3' and 5' fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544947 | PMC |
| http://dx.doi.org/10.1002/cbic.202200281 | DOI Listing |
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