Dataset of response characteristics of H-producing bacteria consortium to β-lactams, aminoglycosides, macrolides, quinolones antibiotics.

Antibiotics on H producing bacteria shall be considered as being one of the critical elements in biological H production utilizing livestock manure as raw resources. Despite the fact that the manure stands a significance role in bio-fermentation, the possibility of antibiotics being contained in excreta shall not be eliminated. Findings of whether the above saying might threaten the safety of bio-H production needs to be further studied. The experiment subjects include: six single and three combined antibiotics were tested and analyzed by the application of the gradient experiment method. Along with the H production rate, CHO content, pH and OD were used to analyze the effects of various antibiotics introduction on the hydrolysis, fermentation and H production. To a further extent, four typical representative samples were selected for biodiversity analysis from the single antibiotic experiment groups. Amounting more than 6000 pieces of data were obtained in a series of experiments. Data suggested that remarkable measure of antibiotics have various degrees of H production inhibition, while some antibiotics, Penicillin G, Streptomycin Sulfate, and their compound antibiotics, could promote the growth of sp. and improve H yield in the contrary. Correspondent to the transition of key metabolic intermediates and end products, the mechanism of each antibiotic type and dose on H production were summarized as follows: the main inhibitory mechanisms were: (1) board-spectrum inhibition, (2) partial inhibition, (3) H consumption enhancement; and the enhancement mechanisms were: (1) enhance the growth of H-producing bacteria, (2) enhanced starch hydrolysis, (3) inhibitory H consumption or release of acid inhibition. Meanwhile, data analysis found that the effect of antibiotics on H producing was not only related to type, but also to dosage. Even one kind of antibiotic may have completely opposite effects on H-producing bacteria under different dosage conditions. Inhibition of H yield was highest with Levofloxacin at 6.15 mg/L, gas production was reduced by 88.77%; and enhancement of H yield was highest with Penicillin G at 7.20 mg/L, the gas production increased by 72.90%.