Detection of bacterial agents causing prostate infection by culture and molecular methods from biopsy specimens.

Background And Objectives: Prostatitis affects about 16% of men in their lifetime and sometimes leading to prostate cancer. Bacterial infections are the most common causes of prostatitis. Diagnosis of the causative agents of bacterial prostate infections plays an essential role in timely treating and preventing secondary complications. This study isolated bacterial infectious agents in patients' surgical prostate and evaluated them by routine and molecular microbiological methods.

Materials And Methods: In this cross-sectional study, 72 prostate biopsy specimens were collected from the Orology Departmen of hospitals of Qazvin University of Medical Sciences. All samples were cultured in aerobic and anaerobic conditions. Antibiotic susceptibility test by Kirby-Bauer standard method was performed for all isolated bacteria. In addition, all isolated bacteria were identified using 16S rDNA PCR and sanger sequencing methods. Also, TaqMan real-time PCR was applied to detect and

Results: In conventional culture method, out of 18 positive samples, 15 samples (83.3%) were Gram-negative bacteria and 3 samples (16.6%) were Gram-positive bacteria, containing (55.5%), (11.1%), (5.5%), (11.1%), (11.1%), and (5.5%). The results of molecular identification methods were the same as conventional culture results. Also, four patients were , and three patients were positive for .

Conclusion: Most bacteria isolated from prostate specimens belonged to the family, especially and were cocci isolated in the specimens too. Also, and were identified in prostatitis.