Multielement (H, C, N, O) stable isotope ratio analysis was used to discriminate the geographical origin of velvet antlers (VAs) of deer from ten provinces in China. Ratios of H/H, C/C, N/N, and O/O in the VA samples were measured using isotope ratio mass spectrometry. The results showed that there were highly significant differences in the mean isotopic values and in four isotopic ratios between VA samples from the ten provinces. The most significant difference among the four isotope ratios was in H ratio of VA samples; regions with a more humid climate and higher average ambient temperatures had higher H ratios than those with dry climates and lower temperatures. These results demonstrate that the multiple stable isotopic ratio approach is a powerful tool to help trace the geographical origin of VAs, and could be adopted by government officials to help protect consumer interests from improper labeling in VA markets.
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http://dx.doi.org/10.1039/d2ra02649j | DOI Listing |
Mol Phylogenet Evol
October 2024
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Zoologia, Laboratório de Sistemática e Evolução de Ácaros Acariformes, Av. Antônio Carlos, 6627, Pampulha, Belo Horizonte, MG 31270-901, Brazil; Tyumen State University, 6 Volodarskogo Str., 625003 Tyumen, Russia.
J Nat Med
September 2023
Department of Forensic Medicine, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan.
In this study, we developed a convenient and easy-to-use origin identification method for antler velvets based on a simple DNA extraction technique and single-stranded tag hybridization chromatographic printed-array strip (STH-PAS). The primer sets used to detect Cervus elaphus, Rangifer tarandus, and 12S rRNA did not engage in non-specific reactions such as primer dimer formation. In both the triplex and singleplex assays, the sensitivity was < 1 ng DNA.
View Article and Find Full Text PDFCommun Biol
April 2023
Department of Zoology, Institute of Biology, University of Kassel, Heinrich-Plett-Straße 40, D-34132, Kassel, Germany.
An antagonistic hemolymph-muscular system is essential for soft-bodied invertebrates. Many ecdysozoans (molting animals) possess neither a heart nor a vascular or circulatory system, whereas most arthropods exhibit a well-developed circulatory system. How did this system evolve and how was it subsequently modified in panarthropod lineages? As the closest relatives of arthropods and tardigrades, onychophorans (velvet worms) represent a key group for addressing this question.
View Article and Find Full Text PDFGenome Biol Evol
March 2023
Museum of Comparative Zoology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts.
Genome assemblies are growing at an exponential rate and have proved indispensable for studying evolution but the effort has been biased toward vertebrates and arthropods with a particular focus on insects. Onychophora or velvet worms are an ancient group of cryptic, soil dwelling worms noted for their unique mode of prey capture, biogeographic patterns, and diversity of reproductive strategies. They constitute a poorly understood phylum of exclusively terrestrial animals that is sister group to arthropods.
View Article and Find Full Text PDFInt J Legal Med
March 2023
Department of Forensic Medicine, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-Ku, Tokyo, 113-8421, Japan.
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