Determination of RNA-ligand interactions with the photoaffinity platform PEARL-seq.

Methods

Arrakis Therapeutics, 828 Winter Street, Waltham, MA, USA. Electronic address:

Published: September 2022

In the development of therapeutics, it is important to establish engagement of a compound to its intended target and identify other targets it binds to. Methods for demonstrating target engagement in the growing field of RNA-targeted therapeutics are therefore needed. We present a detailed protocol for Photoaffinity Evaluation of RNA Ligation-Sequencing (PEARL-seq), a platform for determining interactions between small molecule ligands and their target RNA(s). PEARL-seq allows detection of binding and crosslinking events with single nucleotide resolution and allows measurement of enrichment of the target RNA relative to all other RNAs. PEARL-seq is a valuable tool in the effort to verify bona fide RNA-ligand interactions.

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Source
http://dx.doi.org/10.1016/j.ymeth.2022.06.009DOI Listing

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