AI Article Synopsis
- Escherichia coli ST131, especially its H30Rx, H30R1, and C1-M27 subclones, is a major cause of extraintestinal infections and contributes significantly to ESBL-producing infections.
- A study compared E. coli urine isolates from two hospitals in Minneapolis and Boston, finding similar phylogenetic groups and ST131 prevalence but a unique presence of the C1-M27 subclone in Boston.
- Boston isolates showed higher rates of ESBL production, increased virulence gene scores, and specific ESBL genes, highlighting the need for further surveillance of the C1-M27 subclone in the U.S.
Article Abstract
Background: Escherichia coli sequence type (ST) ST131, with its emergent resistance-associated H30Rx, H30R1, and C1-M27 clonal subsets, accounts for the greatest share of extraintestinal E. coli infections and most extended-spectrum β-lactamase (ESBL)-producing E. coli.
Methods: We characterized and compared consecutive E. coli urine isolates from two geographically distinct medical centers in Minneapolis, Minnesota (n = 172) and Boston, Massachusetts (n = 143) for ESBL phenotype, CTX-M-type ESBL genes, phylogenetic groups, selected ST131 subclones, and 40 extraintestinal virulence genes.
Results: Whereas the Boston vs. Minneapolis isolates had a similar prevalence of phylogenetic groups (mainly B2: 79% vs 73%), ST131 (34% vs 28%), H30 (28% vs 21%), and H30Rx (6% vs 5%), the emerging C1-M27 subclone occurred uniquely among Boston (6%) isolates. ESBL production was more prevalent among Boston isolates (15% vs 8%) and among ST131 isolates. Identified ESBL genes included bla (Boston only) and bla. Ciprofloxacin resistance was ST131-associated and similarly prevalent across centers. Boston isolates had higher virulence gene scores.
Conclusions: Despite numerous similarities to Minneapolis isolates, Boston ST131 isolates demonstrated more prevalent ESBL production, higher virulence gene scores, and, uniquely, the C1-M27 subclone and bla. Broader surveillance is needed to define the prevalence of ST131's globally successful C1-M27 subclone across the U.S.
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| http://dx.doi.org/10.1016/j.ajic.2022.06.019 | DOI Listing |
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Article Synopsis
- Escherichia coli ST131, especially its H30Rx, H30R1, and C1-M27 subclones, is a major cause of extraintestinal infections and contributes significantly to ESBL-producing infections.
- A study compared E. coli urine isolates from two hospitals in Minneapolis and Boston, finding similar phylogenetic groups and ST131 prevalence but a unique presence of the C1-M27 subclone in Boston.
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