Background: The spread of carbapenem-resistant (CRE) strains has caused treatment failure and is a worldwide threat to public health. However, there are limited reports on the prevalence of carbapenemase-producing (CPE) in aquatic environments and its association with clinical isolates. This study aimed to investigate the prevalence of CPE in a stream environment and its genetic relationship with clinical isolates in Korea.
Methods: A total of 4,582 water samples were collected from 94 streams. Multiplex PCR and sequencing were used to detect and identify six carbapenemase genes. Multi-locus sequence typing (MLST) was performed to investigate the genetic relatedness between the environmental strains and clinical isolates.
Results: A total of 133 CRE strains were isolated from the streams. was the most common CRE (45.9%), followed by complex (29.3%), (13.5%), (5.3%), and (2.3%). Ninety (67.7%) isolates carried carbapenemase genes. carbapenemase-2 (36.7%) and New Delhi metallo-β-lactamase-5 (32.2%) were the common carbapenemases detected. Sequence type (ST)307 and ST11 strains harboring the gene were the most prevalent in stream and patient samples.
Conclusion: CPE was highly prevalent in streams and closely related to the isolates obtained from patients. Therefore, continuous monitoring of stream environments is required to control the spread of carbapenem resistance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218686 | PMC |
| http://dx.doi.org/10.3389/fmicb.2022.923979 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: The prevalence of carbapenem-resistant (CRE) has emerged as a serious public health problem worldwide, and the data on the fecal carriage of CRE strains in hospitalized children remain limited. This study aimed to investigate the molecular characteristics of intestinal colonization of CRE in hospitalized children in Shandong, China.
Methods: A retrospective study was conducted from August to November 2023.
Distribution and Antibiotic Resistance Analysis of 13,048 Clinically Common Isolates.
Infect Drug Resist
February 2025
Department of Clinical Laboratory, Beijing Aerospace General Hospital, Beijing, People's Republic of China.
Objective: This study investigated the distribution and antibiotic resistance profiles of common bacteria isolated from clinical specimens at a hospital's microbiology laboratory between 2020 and 2022.
Methods: A retrospective analysis was conducted on microbial culture results from clinical specimens collected over three years, including sample types, departmental distribution, pathogen species, and resistance profiles.
Results: A total of 13,048 unique pathogenic strains were isolated, predominantly from respiratory and urine specimens.
Comparison of Sprague Dawley with Long Evans rats on a battery of widely used neurobehavioral tests.
Physiol Behav
February 2025
Neuroscience Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH 45267; Department of Pediatrics, University of Cincinnati College of Medicine and Division of Neurology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA 45229. Electronic address:
Genetic knockout (KO) models are valuable tools for understanding biological functions and disease mechanisms. KO models in rats have fallen behind those in mice which limits advances in areas where rats have advantages because they can perform more complex cognitive tasks. Two widely used rat strains are Sprague Dawley (SD) and Long Evans (LE).
View Article and Find Full Text PDFCnRed: Efficient, Marker-free Genome Engineering of H16 by Adapted Lambda Red Recombineering.
ACS Synth Biol
February 2025
Austrian Centre of Industrial Biotechnology, acib GmbH, 8010 Graz, Austria.
Due to its ability to utilize carbon dioxide, native intracellular accumulation of bioplastic precursors, and a high protein content, the bacterium offers potential solutions for social problems tackled by modern biotechnology. Yet, engineering of high-performing chemolithotrophic production strains has so far been hindered by the lack of adequate genome editing methods. In this work we present the establishment of a lambda Red recombineering system for use in H16.
View Article and Find Full Text PDFUnderstanding and addressing β-lactam resistance mechanisms in gram-negative bacteria in Lebanon: A scoping review.
Heliyon
February 2025
Laboratory Department, Lebanese Hospital-Geitaoui University Medical Center, Beirut, Lebanon.
Background: A growing threat to public health is the worldwide problem of antimicrobial resistance (AMR), in which gram-negative organisms are playing a significant role. Antibiotic abuse and misuse, together with inadequate monitoring and control protocols, have contributed to the emergence of resistant strains. This global scenario prepares us to look more closely at the situation in Lebanon.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!