Chitosan mediated layer-by-layer assembly based graphene oxide decorated surface plasmon resonance biosensor for highly sensitive detection of β-amyloid.

Alzheimer's disease (AD), and its consequent effect primarily clinical dementia, Parkinson's disease dementia, etc. currently bring potential avenues for diagnosis centered on identification of beta-amyloid (Aβ). Unfortunately, techniques engaged in AD core biomarker (Aβ) detection are majorly suffering from poor sensitivity and selectivity. Thus, we fabricated graphene oxide (GO) surface decorated chitosan (CS) mediated layer-by-layer (LbL) assembly based surface plasmon resonance (SPR) biosensor for highly sensitive and selective recognition of Aβ. Briefly, silver nanoparticles (AgNPs) and GO synthesis were achieved through a greener approach. LbL assembly was designed using CS and polystyrene sulphonate (PSS) on surface of AgNPs (AgNPs-CS-PSS-CS) and then antibodies of Aβ (anti-Aβ) were fixed on LbL assembly (AgNPs-CS-PSS-CS@anti-Aβ). Herein, amine functionality of CS offers a plethora of sites for anti-Aβ antibody immobilization that gives specific direction, high selectivity, and an adequate amount of antibody immobilization. For fabrication, synthesized GO was immobilized on an amine-modified gold-coated sensor chip via carbodiimide chemistry followed by AgNPs-CS-PSS-CS@anti-Aβ immobilization on an activated GO surface. Inimitable features of LbL assembly showed improved selectivity towards Aβ peptide whereas utilization of affinity biotransducer with a combination of plasmonic and non-plasmonic nanomaterial improved sensitivity and selectivity. Consequently, linearity range and limit of detection (LOD) of Aβ antigens were found to be 2 fg/mL to 400 ng/mL and 1.21 fg/mL, respectively. Moreover, analysis of Aβ in AD-induced rats confirmed the real-time-applicability of the designed SPR biosensor. Hence, GO surface decorated AgNPs-CS-PSS-CS@anti-Aβ mediated SPR biosensor would provide a novel approach for exceptionally sensitive and selective Aβ detection.