A simple uracil-appended fluorescent sensor (1) has been developed by one pot reaction and characterized by using common spectroscopic methods such as UV-vis, Fluorescence, HRMS and FT-IR analyses. Upon addition of various metal ions to the CHCN solution of sensor 1, the fluorescence was quenched in the presence of Cu / Hg ions. The limit of detection for Cu and Hg was calculated to be 3.31 and 0.316 µM, respectively. Further, the sensor was applied for real-life applications in the determination of Vitamin B (riboflavin) and its presence in milk products. With the incorporation of different sources of vitamin-B to acetonitrile solution of it, there was discernible fluorescence enhancement only in the presence of vitamin B. Also, it has been successfully applied for the detection of Vitamin B (riboflavin) in milk and curd. Moreover, based on the fluorescent color changes, the sensor was utilized for invisible ink applications.
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