Diacylglycerol acyltransferase 2 promotes the adipogenesis of intramuscular preadipocytes in goat.

Diacylglycerol acyltransferase 2 (DGAT2) is the key enzyme that catalyzes the last step of triglyceride synthesis. However, its role in intramuscular fat (IMF) deposition in goat remains unclear. The purpose of this study was to explore the role of DGAT2 in regulating goat IMF deposition. In the present study, the expression of was highest in goat triceps brachii, and highest on the first day after oleic acid induction in goat intramuscular preadipocytes. The overexpression of promoted the accumulation of lipid droplets and triglyceride synthesis, accompanied by the expression upregulation of , , and significantly, and expression downregulation of , , , , and significantly. In contrast, the silencing of decreased the accumulation of lipid droplets, inhibited the expression of , , , , , , , , significantly, and enhanced that of significantly. Overall, these data underscore DGAT2 may play a potentially important role in lipid droplets formation and triglyceride accumulation, so as to maintain intramuscular fat deposition, beyond triglyceride synthesis in goat.