AI Article Synopsis
- - The study investigates the relationship between antimicrobial resistance and amino acid mutations in penicillin-binding proteins of Streptococcus pneumoniae, as well as characterizing penicillin-nonsusceptible isolates in Iran through molecular methods.
- - Out of 149 tested isolates, 53 were identified as penicillin-nonsusceptible, revealing significant percentages of resistance to other antibiotics like ceftriaxone and cefotaxime, along with various serotypes and genotypes.
- - To combat rising antimicrobial resistance, the research suggests that broader vaccination efforts, particularly using the 13-valent pneumococcal conjugate vaccine, could effectively lower the incidence of severe invasive pneumococcal diseases.
Article Abstract
Background: Given the significant role of penicillin-nonsusceptible Streptococcus pneumoniae in inducing severe infectious diseases, identifying serotypes and genotypes that can mediate antimicrobial resistance has become a pillar of treatment strategies. This study aims to determine the correlation between the minimum inhibitory concentration of antimicrobial agents and amino acid mutations in penicillin-binding proteins. Moreover, molecular serotyping and multiple-locus variable number tandem repeat analysis typing were first-ever performed to characterize the invasive penicillin-nonsusceptible S. pneumoniae isolates in Iran.
Methods: Of 149 isolates, antimicrobial susceptibility tests were performed against penicillin, ceftriaxone, and cefotaxime by the MIC Test Strip, and sequence analysis of the pbp genes was performed through PCR-sequencing method. All penicillin-nonsusceptible S. pneumoniae isolates were serotyped and genotyped by sequential multiplex PCR and multiple-locus variable-number tandem repeat analysis, respectively.
Results: Among pneumococcal isolates, 53 isolates were classified as penicillin-nonsusceptible S. pneumoniae, of which 38 (71.7%) and 15 (28.3%) were resistant and intermediate to penicillin, respectively. Furthermore, ceftriaxone- and cefotaxime-nonsusceptible pneumococci constituted 33 (62.2%) and 29 cases (54.7%), respectively. Of note, there were 8 and 41 different serotypes and multiple-locus variable-number tandem repeat analysis types, respectively.
Conclusions: Due to the increasing resistance to antimicrobial agents, the most efficient approach to preventing pneumococcal infection mortality as vaccine-preventable diseases is focusing on wide-spectrum vaccination. Based on our findings, the 13-valent pneumococcal conjugate vaccine could considerably reduce the incidence of invasive pneumococcal diseases due to the high rate of serotype coverage.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9396163 | PMC |
| http://dx.doi.org/10.1002/jcla.24566 | DOI Listing |
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