AI Article Synopsis
- - The study investigates the rising prevalence of fungal infections in the maxillofacial area post-COVID-19, emphasizing the importance of accurate diagnosis through histopathological staining techniques.
- - Four types of stains—Periodic Acid-Schiff (PAS), Alcian Blue, Safranin-O, and Gomori's Methenamine Silver (GMS)—were tested for their effectiveness on fungal smears, with a focus on the morphometric characteristics of various fungal organisms.
- - Results showed that Safranin-O was the most effective stain, providing superior clarity and detection of fungal structures compared to the other stains, highlighting its potential for aiding early diagnosis and reducing complications in orofacial fungal infections.
Article Abstract
In the wake of the COVID-19 pandemic, fungal infections of the maxillofacial region have become prevalent, making their accurate diagnosis vital. Histopathological staining remains a simple, cost-effective technique for differentiation and diagnosis of the causative fungal organisms. The present study aims to evaluate the staining efficacy of Periodic Acid-Schiff (PAS), Alcian Blue, Safranin-O and Gomori's Methenamine Silver (GMS) on fungal smears. This research work also attempts to study the morphometric characteristics of , , . , and , 10 smears each, were stained using PAS, Alcian Blue, Safranin-O and GMS. The morphological characteristics and staining efficacy were examined, and semi-quantitative scoring was performed. , and were stained for the first time with Safranin-O. The morphometric traits were then analyzed using an image analysis software. Safranin-O provided the most reliable staining efficacy amongst the stains and optimum morphological definition for all three organisms. Safranin-O was found to be superior to PAS and GMS, ensuring detection of even the most minute mycotic colonies. The hyphae of to be the largest, and the spores and fruiting body of were found to be the largest amongst the three organisms compared. Early and accurate diagnosis of fungal infections can significantly reduce morbidity in orofacial fungal infections.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229114 | PMC |
| http://dx.doi.org/10.3390/microorganisms10061226 | DOI Listing |
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