AI Article Synopsis
- Previous research indicated that Candida albicans strains in chronic vulvovaginal candidosis (cVVC) infections are less virulent compared to those in sporadic infections.
- In this study, scientists examined 18 Candida albicans isolates from women with either sporadic (8 isolates) or chronic (10 isolates) infections, focusing on their behavior in lab tests simulating infections.
- Results showed that sporadic isolates had greater adhesion to human cells and less resistance to phagocytosis by immune cells compared to chronic isolates, suggesting that the ability of chronic isolates to evade immune response may contribute to recurrence of infections.
Article Abstract
Previous studies have revealed that Candida albicans isolates involved in chronic vulvovaginal candidosis (cVVC) phenotypically express less virulent traits than clinical isolates involved in sporadic infections. In this study, we aimed to further explore this finding by studying the behaviour of those same clinical isolates in in-vitro models of infection. Eighteen clinical Candida albicans isolates were collected from women suffering sporadic (eight isolates) or chronic infections (ten isolates). Adhesion to HeLa cells (human cervical cancer epithelial cell line) and resistance to phagocytosis by RAW 264.7 cells (murine macrophages cell line) were tested in-vitro. In addition, phenotypic expression of virulence factors related with either adhesion or resistance to phagocytosis was tested in-vitro. Results indicated that yeast isolates involved in sporadic infection adhered in a higher proportion of HeLa cells than those of chronic infections, which was related with their ability to produce biofilm (p < 0.05). The ability to evade phagocytosis was related to an elevated production of proteases (p < 0.05) by chronic isolates, while sporadic isolates’ resistance to phagocytosis was related to a higher hydrophobicity of cell walls (p < 0.05). We conclude that the evasion of macrophage-mediated phagocytosis related to the production of proteases might be an important factor involved in the recurrence of vulvovaginal candidosis infection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225182 | PMC |
| http://dx.doi.org/10.3390/life12060838 | DOI Listing |
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