AI Article Synopsis

  • * It involved a comparison between 20 healthy controls and 17 ALS patients, revealing distinct metabolic profiles and specific metabolites like citramalate that could serve as biomarkers for ALS.
  • * The study found global dysfunction in ALS patients' muscles, with notable changes in antioxidant enzyme levels and mitochondrial activity, highlighting potential new avenues for diagnosis and prognosis.

Article Abstract

Diverse biomarkers and pathological alterations have been found in muscle of patients with Amyotrophic lateral sclerosis (ALS), but the relation between such alterations and dysfunction in energetic metabolism remains to be investigated. We established the metabolome of muscle and serum of ALS patients and correlated these findings with the clinical status and pathological alterations observed in the muscle. We obtained data from 20 controls and 17 ALS patients (disease duration: 9.4 ± 6.8 months). Multivariate metabolomics analysis identified a distinct serum metabolome for ALS compared to controls (p-CV-ANOVA < 0.035) and revealed an excellent discriminant profile for muscle metabolome (p-CV-ANOVA < 0.0012). Citramalate was discriminant for both muscle and serum. High lauroylcarnitine levels in muscle were associated with low Forced Vital Capacity. Transcriptomics analysis of key antioxidant enzymes showed an upregulation of SOD3 (p = 0.0017) and GLRX2(1) (p = 0.0022) in ALS muscle. Analysis of mitochondrial enzymatic activity in muscle revealed higher complex II/CS (p = 0.04) and lower LDH (p = 0.03) activity in ALS than in controls. Our study showed, for the first time, a global dysfunction in the muscle of early-stage ALS patients. Furthermore, we identified novel metabolites to be employed as biomarkers for diagnosis and prognosis of ALS patients.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220134PMC
http://dx.doi.org/10.3390/biomedicines10061307DOI Listing

Publication Analysis

Top Keywords

als patients
16
pathological alterations
12
muscle
10
alterations muscle
8
muscle patients
8
amyotrophic lateral
8
lateral sclerosis
8
als
8
muscle serum
8
patients
6

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!