Trichomoniasis, the most common non-viral sexually transmitted infection worldwide, is caused by the protozoon The 5- nitroimidazole drugs, of which metronidazole is the most prescribed, are the only effective drugs to treat trichomoniasis. Resistance against metronidazole is increasingly reported among isolates. can establish an endosymbiosis with two species, and Mycoplasma girerdii, whose presence has been demonstrated to influence several aspects of the protozoan pathobiology. The role of in resistance to metronidazole is controversial, while the influence of M. girerdii has never been investigated. In this work, we investigate the possible correlation between the presence of M. girerdii and/or and the in vitro drug susceptibility in a large group of isolated in Italy and in Vietnam. We also evaluated, via RNA-seq analysis, the expression of protozoan genes involved in metronidazole resistance in a set of syngenic strains, differing only for the presence/absence of the two . Our results show that the presence of significantly increases the sensitivity to metronidazole in and affects gene expression. On the contrary, the symbiosis with Mycoplasma girerdii seems to have no effect on metronidazole resistance in .
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| http://dx.doi.org/10.3390/antibiotics11060812 | DOI Listing |
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