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Improved Hematology Analysis Based on Microfluidic Impedance Spectroscopy: Erythrocyte Orientation and Anisotropic Dielectric Properties of Flowing Blood.
Anal Chem
January 2025
School of Mechanical and Robotics Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
Electrochemical impedance spectroscopy has great potential for laboratory blood tests. The overall aim of this study is to develop a microfluidic sensor for determining the physical properties and hematological parameters of blood based on its dielectric spectra. Impedance was measured in flowing blood to prevent aggregation and sedimentation at frequencies between 40 Hz and 110 MHz.
View Article and Find Full Text PDFProtocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry.
STAR Protoc
January 2025
Heinz-Nixdorf-Chair of Biomedical Electronics, TranslaTUM, School of Computation, Information and Technology, TUM, Germany; Munich Institute of Biomedical Engineering, TUM, Germany. Electronic address:
Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement.
View Article and Find Full Text PDFEffect of a Novel Food Rich in Miraculin on the Intestinal Microbiome of Malnourished Patients with Cancer and Dysgeusia.
Nutrients
January 2025
Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 18071 Granada, Spain.
Background/objectives: Dysgeusia contributes to malnutrition and worsens the quality of life of patients with cancer. Despite the different strategies, there is no effective treatment for patients suffering from taste disorders provided by the pharmaceutical industry. Therefore, we developed a novel strategy for reducing side effects in cancer patients by providing a novel food supplement with the taste-modifying glycoprotein miraculin, which is approved by the European Union, as an adjuvant to medical-nutritional therapy.
View Article and Find Full Text PDFRelationship of Biochemical and Sonographic Markers with Disease Severity in Rosacea Patients Without Cardiovascular Disease.
Life (Basel)
January 2025
Department of Dermatology, New York Medical College, New York, NY 10595, USA.
Rosacea is a chronic inflammatory skin disorder characterized by central facial redness, papulopustular lesions, and occasionally phymatous changes. There is ongoing debate regarding rosacea as a cutaneous disease with systemic inflammatory effects and its associations with cardiovascular diseases. Although the pathogenesis of both atherosclerosis and rosacea demonstrate notable similarities, particularly in the central role of inflammation, significant gaps in understanding these connections remain.
View Article and Find Full Text PDFRegulation of PHD2 by HIF-1α in Erythroid Cells: Insights into Erythropoiesis Under Hypoxia.
Int J Mol Sci
January 2025
Research Center for High Altitude Medicine, Qinghai University, Xining 810016, China.
The hypoxia-inducible factor (HIF) pathway has been demonstrated to play a pivotal role in the process of high-altitude adaptation. PHD2, a key regulator of the HIF pathway, has been found to be associated with erythropoiesis. However, the relationship between changes in Phd2 abundance and erythroid differentiation under hypoxic conditions remains to be elucidated.
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