AI Article Synopsis
- Electroactive bacterial biofilms can integrate living cells and electronic components, but controlling their geometry on electrodes has been difficult.
- A new lithographic method was created to precisely pattern these biofilms by manipulating a specific protein expression using blue light.
- This method allowed for adjustable conductivity based on the biofilm's pattern size and confirmed theoretical models about how electrons move through the living biofilms, paving the way for advancements in bioelectronics.
Article Abstract
Electroactive bacterial biofilms can function as living biomaterials that merge the functionality of living cells with electronic components. However, the development of such advanced living electronics has been challenged by the inability to control the geometry of electroactive biofilms relative to solid-state electrodes. Here, we developed a lithographic strategy to pattern conductive biofilms of by controlling aggregation protein CdrAB expression with a blue light-induced genetic circuit. This controlled deposition enabled biofilm patterning on transparent electrode surfaces, and electrochemical measurements allowed us to both demonstrate tunable conduction dependent on pattern size and quantify the intrinsic conductivity of the living biofilms. The intrinsic biofilm conductivity measurements enabled us to experimentally confirm predictions based on simulations of a recently proposed collision-exchange electron transport mechanism. Overall, we developed a facile technique for controlling electroactive biofilm formation on electrodes, with implications for both studying and harnessing bioelectronics.
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| http://dx.doi.org/10.1021/acssynbio.2c00024 | DOI Listing |
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