This work analyses the adhesion of flagellated microalgae to seven surfaces that have different water adhesion tension characteristics. and , were cultivated in batch and fed-batch mode at four nitrogen/phosphorus (N/P) ratios (from 1.29 to 70) and subjected to four irradiance levels (50, 100, 200 and 400 µE·s·m) at 23 °C. Cell adhesion was greater in and a higher biomass concentration was obtained for this strain, reaching 2 g·L compared to 1 g·L for . The adhesion of cells and exopolymeric substances was measured upon the batch and the first fed-batch reaching the stationary growth phase, observing a direct correlation between them and inversely to biomass generation in the cultures. The protein adhesion data for the different materials are comparable to those for cell adhesion coinciding with minimums of Baier's theory and Vogler. It is observed displacements in the curves as a function of the irradiance level.
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http://dx.doi.org/10.1080/08927014.2022.2089564 | DOI Listing |
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
VTT Technical Research Centre of Finland Ltd, Tietotie 2, P.O. Box 1000, 02044 Espoo, Finland.
This study elucidates the utility and efficacy of UV-visible spectroscopy for the detection and characterization of biological contaminants within microalgae cultures, augmented by machine learning algorithms. Biological contamination, exemplified by flagellates and rotifers, poses a significant concern due to its potential to rapidly devastate entire cultures, thus jeopardizing commercial viability. Conventional analytical methods for monitoring contamination, such as microscopy and cytometry, are often labor-intensive, reliant on specialized expertise for microorganism identification, and may lack specificity in discerning the nature of contamination, impeding timely intervention.
View Article and Find Full Text PDFJ Chromatogr A
January 2025
School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China; Hebei Key Laboratory of Micro-Nano Precision Optical Sensing and Measurement Technology, Qinhuangdao 066004, China. Electronic address:
Flagellate microalgae play an increasingly significant role in environmental management and biotechnology for valuable bioproducts, excellent photosynthetic capability, and autonomic movement. However, multiple flagellate microalgae practically live together in the ocean and lake areas, and they are susceptible to contamination as a result of improper operations. Enthused by these aspects, we develop a reliable inertial microfluidic method to overcome the influence of flagella movement and non-spherical shape on the alignment and isolation of target flagellate microalgae.
View Article and Find Full Text PDFEur J Protistol
August 2024
University of Cologne, Institute of Zoology, General Ecology, 50674 Cologne, Germany. Electronic address:
Protists can endure challenging environments sustaining key ecosystem processes of the microbial food webs even under aridic or hypersaline conditions. We studied the diversity of protists at different latitudes of the Atacama Desert by massive sequencing of the hypervariable region V9 of the 18S rRNA gene from soils and microbial mats collected in the Andes. The main protist groups in soils detected in active stage through cDNA were cercozoans, ciliates, and kinetoplastids, while the diversity of protists was higher including diatoms and amoebae in the microbial mat detected solely through DNA.
View Article and Find Full Text PDFEur J Protistol
February 2024
Institute of Cytology, Russian Academy of Sciences, Saint Petersburg 194064, Russia.
Rhizochromulina marina is a unicellular amoeboid alga capable of forming flagellate cells; it is a single validly named species in the genus. Besides, there are numerous environmental sequences and undescribed strains designated as Rhizochromulina sp. or R.
View Article and Find Full Text PDFEur J Protistol
October 2023
University of Cologne, Institute of Zoology, General Ecology, 50674 Cologne, Germany. Electronic address:
Cercozoans and heterolobose amoebae are found across terrestrial habitats where they feed on other unicellular microbes, including bacteria, fungi and microalgae. They constitute a significant fraction of soil ecosystems and are integral members of plant microbiota. Here, we present the results on the isolation of protozoans from the rhizosphere and phyllosphere of Browningia candelaris (Meyen) in the Andean Altiplano and Eulychnia taltalensis (F.
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