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Fractionation Protocol of Marine Metabolites. | LitMetric

Fractionation Protocol of Marine Metabolites.

Methods Mol Biol

National Research Council of Italy, Institute of Biomolecular Chemistry, Pozzuoli, Naples, Italy.

Published: June 2022

Marine organisms are well known for their capability to produce chemically unique and biological relevant small molecules. However, marine extracts are complex mixtures of compounds with a huge presence of salts. Thus, biological screening and chemical analysis of marine extracts pose specific technical constraints and require adequate sample preparation. We have developed an automated solid-phase extraction (SPE)-based method to desalt marine extracts and recover metabolites, adapt to be integrated in platform of high-throughput screening. The procedure uses a poly(styrene-divynylbenzene)-based support and a stepwise organic solvent elution herein described.

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http://dx.doi.org/10.1007/978-1-0716-2313-8_16DOI Listing

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