Molecular Characterization of Predominant Serotypes, Drug Resistance, and Virulence Genes of Isolates From East China.

is a common diplococcus pathogen found worldwide. The characterization of predominant serotypes, drug resistance, and virulence genes of isolates prevailing in different areas and countries is clinically important for choice of antibiotics and improvement of vaccines. In this study, pneumonia (78.7%) and meningitis (37.0%) were the predominant diseases observed in the 282 (children) and 27 (adults) -infected patients ( < 0.05) from seven hospitals in different areas of East China. Of the 309 pneumococcal isolates, 90.3% were classified by PCR into 15 serotypes, with serotypes 19F (27.2%) and the 6A/B (19.1%) being most predominant ( < 0.05). Importantly, serotypes 15A and 15B/C combined for a total of 10.4% of the isolates, but these serotypes are not included in the 13-valent pneumococcal capsule conjugate vaccine used in China. Antimicrobial susceptibility analysis by the -test showed that >95% of the 309 pneumococcal isolates were susceptible to moxifloxacin and levofloxacin, as well as 18.4, 85.8, and 81.6% of the isolates displayed susceptibility to penicillin, cefotaxime, and imipenem, respectively. A significant correlation between the prevalence of predominant serotypes and their penicillin resistance was observed ( < 0.05). In particular, >95% of all the pneumococcal isolates showed resistance to erythromycin and azithromycin. Of the nine detected virulence genes, the , , , and were the most common with 95-100% positive rates in the 309 pneumococcal isolates, while the and genes displayed a significant correlation with pneumococcal bacteremia and meningitis ( < 0.05). Overall, our data suggested that the predominant serotypes, drug resistance, and virulence genes of the isolates prevailing in East China are distinct from those observed in other areas of China and adjacent countries.