AI Article Synopsis
- - Membrane contact sites play a crucial role in communication between different compartments within cells, especially in mitochondria.
- - The text introduces a protocol to create a synthetic bridge, called MitoT, that connects the inner and outer mitochondrial membranes, enhancing the function of the MICOS complex.
- - This method is beneficial for studying the roles of the MICOS complex and related proteins in cultured mammalian cells, as detailed in the referenced study by Viana et al. (2021).
Article Abstract
Membrane contact sites are recognized as critical means of intercompartmental communication. Here, we describe a protocol for engineering and validating a synthetic bridge between the inner and outer mitochondrial membranes to support functioning of the endogenous mitochondrial contact site and cristae organizing system (MICOS). A chimeric protein, MitoT, is stably expressed in cultured mammalian cells to bridge the mitochondrial membranes. This approach can be a valuable tool to study the function of the MICOS complex and associated proteins. For complete details on the use and execution of this protocol, please refer to Viana et al. (2021).
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204730 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101454 | DOI Listing |
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