This study illustrated the texture changes of -inoculated during refrigerated storage with the exogenous addition of SS-128. The group inoculated with SS-128 had an improved texture compared with that inoculated with the -mutant group (Δ). Proteomics were conducted to analyze the protein alterations in and supernatant, respectively. During storage, many texture-related proteins, including myosin heavy chain and beta-actin, were maintained due to . Some endogenous enzymes related to the energy metabolism and hydrolysis of were downregulated. The -induced interaction with showed significant changes in the expression of some critical enzymes and pathways. The ATP-dependent zinc metalloprotease FtsH and protease subunit HslV were downregulated, and the oxidative phosphorylation and glycosaminoglycan degradation pathways in were inhibited, resulting in the slow deterioration of . By exploring the mechanism underlying SS-128-led manipulation of the metabolism of spoilage bacteria, we clarified the texture maintenance mechanism of in SS-128, providing theoretical evidence for SS-128 application in food preservation.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195002 | PMC |
http://dx.doi.org/10.3389/fmicb.2022.892788 | DOI Listing |
Int J Food Microbiol
March 2023
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong Province 266003, China; Qingdao Engineering Research Center for Preservation Technology of Marine Foods, Qingdao, Shandong Province 266003, China. Electronic address:
The biofilm clustered with putrefying microorganisms and seafood pathogens could cover the surface of aquatic products that pose a risk to cross-contaminating food products or even human health. Fighting biofilms triggers synchronous communication associated with microbial consortia to regulate their developmental processes, and the enhancement of the quorum sensing system in Lactiplantibacillus plantarum can serve as an updated starting point for antibiofilm-forming strategies. Our results showed that the exogenous 25 mM L-cysteine induced a significant strengthening in the AI-2/LuxS system of Lactiplantibacillus plantarum SS-128 along with a stronger bacteriostatic ability, resulting in an effective inhibition of biofilms formed by the simplified microbial consortia constructed by Vibrio parahaemolyticus and Shewanella putrefaciens grown on shrimp and squid surfaces.
View Article and Find Full Text PDFFood Res Int
November 2022
College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong Province 266003, China. Electronic address:
Quorum sensing of lactic acid bacteria, mediated by the Autoinducer-2 (AI-2)/LuxS system, positively regulates antibacterial activity, which is an effective strategy for aquatic product preservation. This study revealed that AI-2/LuxS system regulates the nutritional competitiveness of LAB by facilitating membrane transport systems in preservation of vacuum-packaged refrigerated shrimp (VPRS), using Lactiplantibacillus plantarum SS-128 wild-type and the luxS mutant strain. In VPRS preservation, organisms that cause spoilage and total volatile basic nitrogen were significantly lower in the VPRS inoculated with L.
View Article and Find Full Text PDFFoods
July 2022
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Retarding the protein deterioration of shrimp during storage is important for maintaining its quality. SS-128 ( SS-128) is a biocontrol bacterium that can effectively maintain the fresh quality of food. This research establishes a myofibril simulation system and refrigerated control system to explore the impact of SS-128 on the quality and shelf life of refrigerated shrimp ().
View Article and Find Full Text PDFFront Microbiol
May 2022
College of Food Science and Engineering, Ocean University of China, Qingdao, China.
This study illustrated the texture changes of -inoculated during refrigerated storage with the exogenous addition of SS-128. The group inoculated with SS-128 had an improved texture compared with that inoculated with the -mutant group (Δ). Proteomics were conducted to analyze the protein alterations in and supernatant, respectively.
View Article and Find Full Text PDFFoods
February 2022
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
could regulate certain physiological functions through the AI-2/LuxS-mediated quorum sensing (QS) system. To explore the regulation mechanism on the growth characteristics and bacteriostatic ability of SS-128, a mutant was constructed by a two-step homologous recombination. Compared with Δ/SS-128, the metabolites of SS-128 had stronger bacteriostatic ability.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!