Recent developments in the miniaturization of hyperspectral imaging sensors have given rise to the increased use of hyperspectral imagery as the primary data for evaluating spectral unmixing algorithms in applications such as industrial quality control, agriculture, mineral mapping, military, etc. This article presents an ultra-high-resolution hyperspectral imagery dataset for undertaking benchmark studies on spectral unmixing. A terrestrial hyperspectral imager (THI) is used for imaging the target scene with the camera sensor pointing horizontally towards the target scene. The datasets are acquired at various spatial resolutions ranging from 1 mm to 2 cm. The targeted scene contains several paper-based panels, each size of 2 cm x 2 cm and filled with different colours and proportions, glued to a black background board that maintains a distinguishable distance between one another. In addition to the hyperspectral imagery data acquisitions, reference spectral signatures of the candidate mixture materials are obtained by in-situ hyperspectral reflectance measurements using a spectroradiometer. The hyperspectral image acquisition and the in-situ spectral signatures of the target scene are collected under natural illumination conditions. The proposed datasets are designed for undertaking proof-of-the-concept (PoC) studies in spectral unmixing. The datasets are also valuable for evaluating the performance of different statistical and machine learning algorithms for target detection, classification, and sub-pixel classification algorithms.
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http://dx.doi.org/10.1016/j.dib.2022.108331 | DOI Listing |
J Med Imaging (Bellingham)
January 2025
Okaya City Hospital, Division of Diagnostic Pathology, Okaya, Japan.
Purpose: The color of Papanicolaou-stained specimens is a crucial feature in cytology diagnosis. However, the quantification of color using digital images is challenging due to the variations in the staining process and characteristics of imaging equipment. The dye amount estimation of stained specimens is helpful for quantitatively interpreting the color based on a physical model.
View Article and Find Full Text PDFIntroduction: Although the widespread use of screening tests and HPV vaccines for squamous cell carcinoma has led to early detection and treatment, effectiveness is limited for cervical adenocarcinoma. Lobular endocervical glandular hyperplasia (LEGH) corresponds to gastric metaplasia, but is regarded as a pathological condition with subtle morphological abnormalities. LEGH is a benign lesion and a precursor to gastric-type adenocarcinoma.
View Article and Find Full Text PDFAnal Chem
December 2024
Research Center for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin 300071, China.
Due to the complexity of samples and the limitations in spatial resolution, the spectra in hyperspectral imaging (HSI) are generally contributed to by multiple components, making univariate analysis ineffective. Although feature extraction methods have been applied, the chemical meaning of the compressed variables is difficult to interpret, limiting their further applications. An unmixing autoencoder (UAE) was developed in this work for the separation of the mixed spectra in HSI.
View Article and Find Full Text PDFMed Image Anal
December 2024
School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, China; Guangdong Provincial Key Laboratory of Medical Image Processing, Southern Medical University, Guangzhou 510515, China; Guangdong Province Engineering Laboratory for Medical Imaging and Diagnostic Technology, Southern Medical University, Guangzhou 510515, China. Electronic address:
Photoacoustic tomography (PAT), as a novel biomedical imaging technique, is able to capture temporal, spatial and spectral tomographic information from organisms. Organ-level multi-parametric analysis of continuous PAT images are of interest since it enables the quantification of organ specific morphological and functional parameters in small animals. Accurate organ delineation is imperative for organ-level image analysis, yet the low contrast and blurred organ boundaries in PAT images pose challenge for their precise segmentation.
View Article and Find Full Text PDFMolecules
November 2024
Departamento de Biotecnología y Bioquímica, Cinvestav Irapuato, Km. 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato CP 36821, GTO, Mexico.
The alkamide content and specific tissue localization in the cypselae of were investigated using gas chromatography-electron ionization mass spectrometry (GC-EIMS) and multiphoton fluorescence microscopy (MPFM). GC-EIMS analysis identified two olefinic alkamides: affinin (spilanthol) and -2-methylbutyl-2,6,8-decatrienamide. Microscopic analysis revealed that alkamides are localized within the cotyledons, and specifically compartmentalized in lipid bodies, highlighting their spatial organization.
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