AI Article Synopsis
- - The CRISPR-Cas genome editing system is widely used due to its versatility in research and biotechnology, particularly in eukaryotic organisms like yeast.
- - The overview covers tools and methods for efficient, marker-free genome editing in yeast, including techniques like sgRNA design, multiplex editing, and optimizing Cas9 expression.
- - Recent studies are highlighted to showcase potential issues with CRISPR in yeast, such as background mutations that can arise during the editing process.
Article Abstract
The versatility of clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) genome editing makes it a popular tool for many research and biotechnology applications. Recent advancements in genome editing in eukaryotic organisms, like fungi, allow for precise manipulation of genetic information and fine-tuned control of gene expression. Here, we provide an overview of CRISPR genome editing technologies in yeast, with a particular focus on . We describe the tools and methods that have been previously developed for genome editing in and discuss tips and experimental tricks for promoting efficient, marker-free genome editing in this model organism. These include sgRNA design and expression, multiplexing genome editing, optimizing Cas9 expression, allele-specific editing in diploid cells, and understanding the impact of chromatin on genome editing. Finally, we summarize recent studies describing the potential pitfalls of using CRISPR genome targeting in yeast, including the induction of background mutations.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9190257 | PMC |
| http://dx.doi.org/10.3389/fbioe.2022.924914 | DOI Listing |
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