Serum IL-6 as a marker of disease progression in interstitial nephritis.

Objective: To investigate the mechanism of serum interleukin-6 (IL-6) change in disease progression of interstitial nephritis.

Methods: This is a retrospective study. From November 2017 to November 2019, 87 patients with interstitial nephritis treated in our hospital were enrolled and divided into an acute group (n=42) and a chronic group (n=45) based on pathological results of renal biopsies. Forty healthy individuals after physical examination during the same period were enrolled into the reference group. Serum IL-6 levels were determined using the enzyme-linked immunosorbent assay (ELISA).

Results: Among the three groups, patients in the acute group showed the highest IL-6 level (P<0.001). The acute group obtained higher serum advanced oxidation protein products (AOPP) levels and glomerular filtration rate (GFR) than the other two groups (P<0.05). The acute group showed lower levels of CD34 [number of positive microvessels (MVs)/HP], a smaller type III collagen positive area, and a larger type IV collagen positive area than the chronic group (P<0.05). The acute group obtained higher levels of IL-27 and tumor necrosis factor-α (TNF-α) than the chronic group (P<0.001). The acute group had higher levels of serum creatinine (SCr), erythrocyte sedimentation rate (ESR), estimated glomerular filtration rate (eGFR), and 24-hour urine protein quantity (24 h UPQ) than the other groups (P<0.001). The combined detection of serum IL-6, TNF-α, and micro-albumin (mALB) outperformed the stand-alone approach (P<0.05). Serum IL-32 and kidney injury molecule-1 (KIM-1) levels in the acute and chronic group were positively correlated with SCr and 24 h UPQ (P<0.05).

Conclusions: Serum IL-6 shows a great potential as an important marker of disease progression in interstitial nephritis.