Background: Cutaneous T-cell lymphoma (CTCL) is highly heterogeneous, and its prognosis is closely related to the disease stage. The tumor microenvironment (TME) is an important component of tumor tissue, driving cancer cell growth, progression, and metastasis. However, the diagnostic value of TME in CTCL has not yet been studied in-depth. To date, no study has performed a comprehensive evaluation of the significance of the TME in CTCL.
Methods: Using xCell methods based on bulk RNA sequencing data, we inferred immune cell fraction in the TME in 126 patients and assessed the prognostic importance of immune cells. Consensus clustering was performed to determine the TME subtypes and characterize the transcriptome of each subtype. Based on the TME subtypes, we established the disease progression model using random forest algorithms and logistic regression. The efficacy of the model was examined using an additional 49-patient cohort. Finally, we validated our finding at the protein level using immunochemistry in a 16-patient cohort.
Results: Patients with advanced CTCL presented with a more active immunity overall than those with early stage. Random forest algorithms revealed that the immune cells CD4, macrophages, and dendritic cells (DCs) were the most effective prognosis predictors. Therefore, we constructed a risk model using logistic regression based on these immune cells. The TME score could be used to effectively predict disease conditions in three datasets with the AUC of 0.9414, 0.7912, and 0.7665, respectively. Immunochemistry at the protein level revealed that helper T cells and the macrophage markers CD4 and CD68 could successfully distinguish different CTCL stages in patients, whereas the DC marker langerin showed no change with disease progression.
Conclusion: We found advanced-stage CTCL was associated with an active immune microenvironment, and the immune signatures CD4 and CD68 showed a relatively high accuracy in predicting CTCL disease progression.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185059 | PMC |
Publication Analysis
Top Keywords
Similar Publications
PCDHGA10 as a potential prognostic biomarker and correlated with immune infiltration in gastric cancer.
Front Immunol
December 2024
Department of General Surgery, Medical School of Nantong University, & Department of Gastrointestinal Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Background: Gastric cancer (GC) is one of the most common malignant tumors and is associated with poor prognosis. To improve the prognosis of GC patients, an effective immune-related prognostic biomarker is urgent. Here, we aim to explore the correlation between the expression of procalcitonin gamma subfamily A, 10 (PCDHGA10) and clinicopathological characteristics, especially its relation with tumor-infiltrating immune cells (TILs) in GC.
View Article and Find Full Text PDFCombined injection of pristane and Bacillus Calmette-Guerin Vaccine successfully establishes a lupus model with atherosclerosis.
Exp Cell Res
December 2024
Institute of Clinical Pharmacology, Key Laboratory of Anti-inflammatory and Immune Medicine (Anhui Medical University), Ministry of Education, Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Anhui Medical University, Anhui, 230032, China; The Third Affiliated Hospital of Anhui Medical University (The First People's Hospital of Hefei), Hefei, 230061, China. Electronic address:
Cardiovascular disease (CVD) induced by atherosclerosis (AS) is the main fatal complication of systemic lupus erythematosus (SLE). Establishing an appropriate animal model of SLE with AS is of great value for investigating the pathogenesis and therapeutic targets of SLE-CVD. In the present work, pristane was injected intraperitoneally into C57BL/6J mice to establish the SLE model and Bacillus Calmette-Guerin Vaccine (BCG) was injected intradermally one month later to enhance immunity and induce AS.
View Article and Find Full Text PDFElevated PD-L1 and PECAM-1 as Diagnostic Biomarkers of Acute Rejection in Lung Transplantation.
Transpl Int
December 2024
Department of Immunology, Second Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czechia.
Article Synopsis
- - Acute cellular rejection (ACR) is common after lung transplants and can lead to chronic lung problems and affect survival rates; diagnosing it can be tricky due to inconsistent interpretations among pathologists.
- - This study explored the use of immunohistochemistry to identify specific markers (like PD-L1 and PECAM-1) in lung tissue samples from lung transplant patients to improve ACR diagnosis.
- - Results showed that PD-L1 levels were higher in patients with ACR, indicating an immune response suppression effort, and there were notable differences in PECAM-1 levels, highlighting these markers’ potential usefulness for detecting ACR.
An immune suppressive tumor microenvironment in primary prostate cancer promotes tumor immune escape.
PLoS One
November 2024
Division of personalised oncology, Walter and Eliza Hall Institute, Melbourne, Australia.
Background: Immunotherapy has demonstrated limited activity in prostate cancer to date. This likely reflects an immune suppressive tumor microenvironment (TME), with previous studies suggesting low PD-L1 expression and a sparse immune cell infiltrate. We aimed to further characterise the immune TME in primary prostate cancer and correlate immune subset densities with clinical outcomes.
View Article and Find Full Text PDFGene Expression Profiling of the Peritumoral Immune Cell Infiltrate of Penile Squamous Cell Carcinomas.
Int J Mol Sci
November 2024
Dr. Senckenberg Institutes of Pathology & Human Genetics, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60596 Frankfurt am Main, Germany.
Article Synopsis
- - Penile carcinomas are uncommon in Europe and their poor prognosis in advanced stages necessitates further research, particularly concerning the role of immune cell infiltrates in tumor behavior.
- - A study analyzed twelve cases of penile squamous cell carcinomas, focusing on their immune cell infiltration patterns using immunohistochemistry and RNA expression profiling, revealing distinct clustering based on immune cell density.
- - The analysis found that increased immune cell infiltration correlated with specific gene upregulation and various immune functions, while no link to HPV infection status was identified, supporting the validity of findings through subsequent immunohistochemical studies.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!