Stroke leads to gut bacterial dysbiosis that impacts the post-stroke outcome. The gut microbiome also contains a high abundance of viruses which might play a crucial role in disease progression and recovery by modulating the metabolism of both host and host's gut bacteria. We presently analyzed the virome composition (viruses and phages) by shotgun metagenomics in the fecal samples obtained at 1 day of reperfusion following transient focal ischemia in adult mice. Viral genomes, viral auxiliary metabolic genes, and viral protein networks were compared between stroke and sham conditions (stroke vs sham, exclusive to sham and exclusive to stroke). Following focal ischemia, abundances of 2 viral taxa decreased, and 5 viral taxa increased compared with the sham. Furthermore, the abundance of Clostridia-like phages and Erysipelatoclostridiaceae-like phages were altered in the stroke compared with the sham cohorts. This is the first report to show that the gut virome responds acutely to stroke.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441728 | PMC |
| http://dx.doi.org/10.1177/0271678X221107702 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Communities, mysteries, and pathophysiologies - Are gut microbes key to a healthy mind?
Brain Behav Immun
January 2025
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Microbial Systems Initiative, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Personalized Nutrition Initiative, University of Illinois at Urbana-Champaign, Urbana, IL, USA. Electronic address:
Benchmarking of a time-saving and scalable protocol for the extraction of DNA from diverse viromes.
PeerJ
January 2025
Department of Microbiology & Immunology, McGill University, Montreal, QC, Canada.
The virome, composed of viruses inhabiting diverse ecosystems, significantly influences microbial community dynamics and host health. The phenol-chloroform DNA extraction protocol for viromes, though effective, is time-intensive and requires the use of multiple toxic chemicals. This study introduces a streamlined, scalable protocol for DNA extraction using a commercially-available kit as an alternative, assessing its performance against the phenol-chloroform method across human fecal, mouse fecal, and soil samples.
View Article and Find Full Text PDFDoes preeclampsia impact the gut microbiota of preterm offspring during early infancy?
J Transl Med
January 2025
Medical Genetic Center, Guangdong Women and Children Hospital, Guangzhou, China.
Preeclampsia (PE) is a pregnancy complication characterized by high blood pressure and organ damage. This study investigates the differences in the gut microbiota between preterm neonates born to mothers with PE and those born to mothers without PE (PR), aiming to understand how maternal health conditions like PE influence neonatal gut microbiota. The early gut microbiota plays a crucial role in neonatal health, and disturbances in its development can have long-term consequences.
View Article and Find Full Text PDFGut virome dysbiosis impairs antitumor immunity and reduces 5-fluorouracil treatment efficacy for colorectal cancer.
Front Oncol
December 2024
Department of Clinical Medicine, Chengdu Medical College, Chengdu, Sichuan, China.
Introduction: Despite the established influence of gut bacteria, the role of the gut virome in modulating colorectal cancer (CRC) patient chemotherapy response remains poorly understood. In this study, we investigated the impact of antiviral (AV) drug-induced gut virome dysbiosis on the efficacy of 5-FU in CRC treatment.
Methods: Using a subcutaneous CRC mouse model, we assessed tumor growth and immune responses following AV treatment, fecal microbiota transplantation (FMT), and 5-FU administration.
Identification and characterization of prophages rich in diversity-generating retroelements.
Microbiol Spectr
December 2024
Department of Genetics, University Medical Center Groningen, Groningen, the Netherlands.
Article Synopsis
- Metagenomics has uncovered a vast diversity of phages in the human gut, but detailed studies on these phages are limited.
- A new bioinformatics method was developed to identify prophages in cultured gut bacteria, leading to the discovery of 15 candidate prophages, with five validated as active.
- The study also found that some active phages had unique genetic features and varied host ranges, while noting a difference in prevalence among inflammatory bowel disease patients compared to healthy individuals, emphasizing the potential of studying prophage dynamics in understanding microbial ecology.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!