Production Ganoderma lucidum extract nanoparticles by expansion of supercritical fluid solution and evaluation of the antioxidant ability.

Due to the growing human tendency to treat with natural substances, fungi such as Ganoderma lucidum can be a good source to meet this need. Effectiveness, ease of use and a rich source of active ingredients such as ganoderic acids have caused G. lucidum to be considered in the pharmaceutical and food industries. In this project, G. lucidum was applied to extraction using supercritical carbon dioxide. Then expansion of supercritical fluid solution (ESS) was used as, novel, repeatable and green method to yield nanoparticles from G.lucidum extract. The response surface method was used to improve the Extraction efficiency, antioxidant activity, and improving the nanoparticles production status. Optimal conditions were observed at the extraction step by setting pressure at 27.5 MPa, dynamic time of 46 min, and modifier volume of 162 μL. The optimum point for the production of nanoparticles was obtained as follows: pressure drop at 25 MPa, 20 min for collection time, and 40° C for temperature. Under these conditions, the size and count were 86.13 nm, and 98, respectively. Nanoparticles were analyzed by FESM and, the DPPH was used for antioxidant activity evaluation. The LC-MS identified various ganoderic acids from G.lucidum that are famous to be highly oxygenated triterpenoids.