Lysobacter chinensis sp. nov., a cellulose-degrading strain isolated from cow dung compost.

A novel bacterial strain, TLK-CK17, was isolated from cow dung compost sample. The strain was Gram-staining negative, non-gliding rods, aerobic, and displayed growth at 15-40 °C (optimally, 35 °C), with 0-5.0% (w/v) NaCl (optimally, 0.5) and at pH 6.5-8.5 (optimally, 7.0-7.5). The assembled genome of strain TLK-CK17 has a total length of 4.3 Mb with a G + C content of 68.2%. According to the genome analysis, strain TLK-CK17 encodes quite a few glycoside hydrolases that may play a role in the degradation of accumulated plant biomass in compost. On the basis 16S rRNA gene sequence analysis, strain TLK-CK17 showed the highest sequence similarity (98.9%) with L. penaei GDMCC 1.1817, followed by L. maris KCTC 42381 (98.3%). Cells contained iso-C, iso-C, and summed feature 9 (comprising C ω9c and/or 10-methyl C), as its major cellular fatty acids (> 10.0%) and ubiquinone-8 as the exclusively respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol prevailed among phospholipids. Based on the phenotypic, genomic and phylogenetic data, strain TLK-CK17 represents a novel species of the genus Lysobacter, for which the name Lysobacter chinensis sp. nov. is proposed, and the type strain is TLK-CK17 (= CCTCC AB2021257 = KCTC 92122).