Background And Aim: The principal cytokines released by the host on infection include pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF). These cytokines were regarded as regulators of the host's response to infection. This study aimed to determine the release of pro-inflammatory cytokines from chicken peripheral blood mononuclear cells (PBMCs) following lipopolysaccharide (LPS) stimulation.
Materials And Methods: Blood samples were collected from six Betong chickens. To isolate PBMCs, density gradient centrifugation was utilized. PBMC culture in RPMI1640 with 10% fetal bovine serum was stimulated with various concentrations of LPS (0, 0.01, 0.1, and 1.0 μg/mL). The production of TNF-α, IL-1β, and IL-6 was determined using an enzyme-linked immunosorbent assay.
Results: When the PBMCs were cultured for 24 h with varying doses of LPS, there was no significant variation in cell viability. TNF-α, IL-1β, and IL-6 levels were measured in Betong chicken PBMC. The release of these cytokines increased considerably as LPS concentration (0.01-1 μg/mL) increased (p<0.05).
Conclusion: studies of the chicken immune response, notably the release of pro-inflammatory cytokines, can be conducted using PBMCs obtained from chicken blood.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178577 | PMC |
| http://dx.doi.org/10.14202/vetworld.2022.885-889 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The primary cilium gene CPLANE1 is required for peripheral nervous system development.
Dev Biol
December 2024
Department of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Switzerland; University Research Priority Program 'Adaptive Brain Circuits in Development and Learning' (URPP AdaBD), University of Zurich, Switzerland. Electronic address:
Ciliopathies are a group of neurodevelopmental disorders characterized by the dysfunction of the primary cilium. This small protrusion from most cells of our body serves as a signaling hub for cell-to-cell communication during development. Cell proliferation, differentiation, migration, and neural circuit formation have been demonstrated to depend on functional primary cilia.
View Article and Find Full Text PDFThe search to understand the development of the chicken immune system: Differences in expression of MHC class I loci and waves of thymocytes as evolutionary relics?
Dev Biol
December 2024
University of Edinburgh, Institute for Immunology and Infection Research, Edinburgh, United Kingdom. Electronic address:
Chickens are renowned as a model for embryogenesis but have also been responsible for crucial advances in virology, cancer research and immunology. However, chickens are best known as a major source of animal protein for human nutrition, with roughly 80 billion chickens alive each year supplying meat and eggs, the vast majority part of a global poultry industry. As a result, avian immunology been studied intensively for over 60 years, and it has become clear that a major genetic locus in chickens determining resistance to infectious disease and response to vaccines is the major histocompatibility complex (MHC).
View Article and Find Full Text PDFSelenium Ameliorates Aluminum Poisoning-Induced Impaired Production of Neutrophil Extracellular Traps in Chicken.
Biol Trace Elem Res
December 2024
School of Life Sciences and Engineering, Foshan University, Foshan, 528225, Guangdong Province, China.
Neutrophil extracellular traps (NETs) are released by neutrophils to modulate the immune response. Aluminum (Al) poisoning is linked to immunotoxicity, and selenium (Se) can maintain immune homeostasis. In this study, we investigated the toxic effects of Al on the release of NETs, the antagonistic effect of Se on Al-induced toxicity, and the potential molecular mechanisms underlying these processes.
View Article and Find Full Text PDFEffects of maternal Escherichia coli lipopolysaccharide exposure on offspring: insights from lncRNA analysis in laying hens.
Poult Sci
November 2024
Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. Electronic address:
Parental living environment significantly impacts on offspring, yet related studies are lacking in livestock and poultry production. The present study found that lipopolysaccharide (LPS, Escherichia coli, 0.2 mg/kg) stimulation in F0 hens led to growth retardation and a decrease in egg-laying rate in the unchallenged F1 hens.
View Article and Find Full Text PDFCross-species single-cell analysis reveals divergence and conservation of peripheral blood mononuclear cells.
BMC Genomics
December 2024
State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, 510642, China.
Background: Single-cell transcriptome sequencing (scRNA-seq) has revolutionized the study of immune cells by overcoming the limitations of traditional antibody-based identification and isolation methods. This advancement allows us to obtain comprehensive gene expression profiles from a diverse array of vertebrate species, facilitating the identification of various cell types. Comparative immunology across vertebrates presents a promising approach to understanding the evolution of immune cell types.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!