AI Article Synopsis
Article Abstract
This study presents the first ultra-high performance supercritical fluid chromatography-diode array detector (UHPSFC-DAD) assay for simultaneous quantitation of secoiridoids, iridoids, xanthones, and xanthone glycosides in L. Separation was reached within 12 min on an Acquity UPC BEH 2-EP column using CO and methanol with 5.5% water as mobile phases. Method validation for nine selected marker compounds (gentisin, isogentisin, swertiamarin, sweroside, gentiopicroside, loganic acid, amarogentin, gentioside, and its isomer) confirmed the assay's sensitivity, linearity, precision, and accuracy. The practical applicability was proven by the analysis of 13 root specimens and 10 commercial liquid preparations (seven liqueurs and three clear spirits). In all root batches, the secoiridoid gentiopicroside dominated (2.1-5.6%) clearly over all other metabolites. In the liqueurs, the metabolite content and distribution were extremely variable: while gentiopicroside was the main compound in four liqueurs, sweroside dominated in one preparation and loganic acid in two others. In contrast, measurable amounts of the metabolites were not detected in any of the examined clear spirits.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228070 | PMC |
| http://dx.doi.org/10.1021/acs.jafc.2c01584 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Analysis of chemical constituents of different processed products of Strychni Semen based on UPLC-Q-TOF-MS technique].
Zhongguo Zhong Yao Za Zhi
November 2024
School of Pharmacy, Jiangxi University of Chinese Medicine Nanchang 330004, China.
This study utilized ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) to rapidly analyze and identify the chemical constituents in five processed products of Strychni Semen(raw, sand-roasted, fried, urine-soaked, and vinegar-processed products). Using PeakView software to extract compound information, 50 chemical components were identified based on retention time, accurate molecular ion peaks, secondary mass spectrometry data, and comparison with reference standards and relevant literature. Specifically, 41 components were identified in raw Strychni Semen, 48 in sand-roasted, 43 in fried, 41 in urine-soaked, and 40 in vinegar-processed products.
View Article and Find Full Text PDFAmplifying Bioactivity of blue honeysuckle (Lonicera caerulea L.) fruit puree through Ultrasonication: Antioxidant and antiproliferative activity.
Ultrason Sonochem
November 2024
Heilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin 150030, PR China; National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, Northeast Agricultural University, Harbin 150030, PR China; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:
Blue honeysuckle (Lonicera caerulea L.) serves as a significant reservoir of polyphenol compounds. This impact of ultrasonication processing on the bioaccessibility of blue honeysuckle fruit puree during in vitro digestion was evaluated.
View Article and Find Full Text PDFGallic acid and loganic acid attenuate amyloid-β oligomer-induced microglia damage via NF-КB signaling pathway.
Neuropharmacology
February 2025
Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xian Yang, Shaanxi Province, 712082, China. Electronic address:
Amyloid β peptide (Aβ) induces neurodegeneration in the early stage of Alzheimer's disease (AD), resulting in neuroinflammation, oxidative damage, and mitochondrial impaired function. These reactions were closely associated with the pathological changes of brain microglia. Therefore, it was crucial to investigate the precise process of neuroinflammation induced by Aβ in microglia and discover therapies to alleviate its harmful consequences.
View Article and Find Full Text PDFIntegrated Transcriptomics and Metabolomics Reveal Key Insights into Iridoid Biosynthesis in Seeds during Germination.
Genes (Basel)
September 2024
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Duthie ex Burk., a key species used in traditional Chinese medicine for treating rheumatic pain and stroke, contains iridoids as its primary active component. However, the biosynthetic mechanisms underlying iridoid production are not fully understood.
View Article and Find Full Text PDFDesign and investigation of novel iridoid-based peptide conjugates for targeting EGFR and its mutants L858R and T790M/L858R/C797S: an in silico study.
Mol Divers
October 2024
Department of Chemistry and Biochemistry, Fordham University, 441 East Fordham Road, Bronx, NY, 10458, USA.
In this work, we designed novel peptide conjugates with plant-based iridoid and lichen-derived depside derivatives to target the wild-type EGFR (WT) and its mutants, L858R and T790M/L858R/C797S triple mutant. These mutations are often expressed in multiple cancers, particularly lung cancer. Specifically, the iridoids included 7-deoxyloganetic acid (7-DGA) and loganic acid (LG), while the depside derivative was sekikaic acid (SK).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!