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Rapid and accurate nanoelectrokinetic diagnosis of drug-resistant bacteria. | LitMetric

Rapid and accurate nanoelectrokinetic diagnosis of drug-resistant bacteria.

Biosens Bioelectron

Department of Electrical and Computer Engineering, Seoul National University, Seoul, 08826, Republic of Korea; Inter-university Semiconductor Research Center,Seoul National University, Seoul, 08826, South Korea; SOFT Foundry Institute, Seoul National University, Seoul, 08826, South Korea. Electronic address:

Published: October 2022

AI Article Synopsis

  • * The newly developed Rapid and Accurate Nanoelectrokinetic Diagnostic System (RANDx) can detect drug-resistant bacteria within just 7 minutes by using a nanoelectrokinetic paper-based device (NEK-PAD) that enhances sample preparation.
  • * RANDx utilizes a unique design that enables significant preconcentration of bacteria and extraction of drug-resistant enzymes, allowing for the detection of bacteria at low concentrations without extra labeling, thus showing promise for use in remote medical settings.

Article Abstract

Increased antimicrobial resistance presents a major threat to public health, and it is a global health problem due to the rapid globalization and transmission of infectious diseases. However, fast and precise diagnosis tool is lacking, and inappropriate antibiotic prescription leads to the unforeseen production of drug-resistant bacteria. Here, we report a Rapid and Accurate Nanoelectrokinetic Diagnostic System (RANDx) for detecting drug-resistant bacteria, which cause a common infectious disease called Urinary Tract Infection (UTI), within 7 min. We develop nanoelectrokinetic paper-based analytic device (NEK-PAD) as a sample prep module of RANDx and obtain >100-fold post-wetting preconcentration by balancing between ion concentration polarization (ICP) and radial imbibition for a constant flow rate. Simultaneously with preconcentration, our cathodic nanochannel design enables NEK-PAD to extract drug-resistant enzymes without denaturation and accelerate enzyme-linked reactions under electrical spontaneous heating at approximately 37 °C. Finally, using a cell phone camera, we detect label-free drug-resistant bacteria as low as 10 cfu/mL, which is higher than clinically required threshold (>10 cfu/mL) by enhancing 1000 times of the limit of detection (LOD) of colorimetric nitrocefin assay. We believe that the RANDx will be an innovative precision medicine tool for UTI and other infectious diseases in limited remote settings.

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Source
http://dx.doi.org/10.1016/j.bios.2022.114350DOI Listing

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