AI Article Synopsis
- The study investigates how Xuesaitong injection (XST), a traditional Chinese medicine, may help treat ischemic stroke by analyzing gene expression and pathways.
- The research identifies 8,066 differentially expressed genes (DEGs), with the MAPK signaling pathway and brain-derived neurotrophic factor (BDNF) being key players in XST's protective effects against cerebral ischemia-reperfusion injury.
- Experimental validation shows that XST enhances BDNF and TrkB levels, and its neuroprotective effects are diminished when BDNF is suppressed, highlighting the importance of the BDNF-TrkB pathway in its mechanism of action.
Article Abstract
Background: Xuesaitong injection (XST), a well-known traditional Chinese patent medicine, has been widely used in the treatment of cardiovascular and cerebrovascular diseases. The exact mechanisms of XST in ischemic stroke remain to be thoroughly elucidated.
Purpose: This study aims to characterize the candidate differentially expressed genes (DEGs) and pathways of XST in ischemic stroke by bioinformatics analysis, and to explore new clues for the underlying mechanisms of XST.
Methods: A dataset (GSE61616) was performed to screen out DEGs for deep analysis. Series Test of Cluster analysis for DEGs was carried out. For all DEGs, Gene Ontology (GO) annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed for visualization. The screened hub gene expression characteristics were verified in middle cerebral artery occlusion (MCAO) rats. In vivo studies have demonstrated the mechanisms of XST against cerebral ischemia-reperfusion (CIR) injury.
Results: A total of 8066 DEGs were screened out and the expression of genes in profile 8 was suggested to have clinical significance. The MAPK signaling pathway was indicated as the most significantly enriched pathway in profile 8. Bdnf was identified as the most significant hub gene according to node degree. Animal experiments demonstrated that XST attenuated CIR injury. XST increased brain-derived neurotrophic factor (BDNF) and its high-affinity receptor tropomyosin-related kinase B (TrkB) levels in MCAO. Furthermore, the knockdown of BDNF by siRNA abolished the in vivo effects of XST on brain injury, neurodegeneration and apoptosis after CIR.
Conclusion: The integrated strategy, based on bioinformatics analyses with experimental verification, provides a novel cellular mechanism by which XST alleviates CIR injury. The BDNF-TrkB pathway was highly thought to play a vital role in the neuroprotective effects of XST.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.phymed.2022.154224 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Random survival forest model for early prediction of Alzheimer's disease conversion in early and late Mild cognitive impairment stages.
PLoS One
December 2024
Department of Mechanical Engineering and Artificial Intelligence, Ajman University, Ajman, United Arab Emirates.
With a clinical trial failure rate of 99.6% for Alzheimer's Disease (AD), early diagnosis is critical. Machine learning (ML) models have shown promising results in early AD prediction, with survival ML models outperforming typical classifiers by providing probabilities of disease progression over time.
View Article and Find Full Text PDFPanax notoginseng saponins promotes angiogenesis after cerebral ischemia-reperfusion injury.
J Ginseng Res
November 2024
Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine (Natural Medicine) and Translational Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Background: Ischemic stroke is a devastating disease that can result in permanent disability and death, and angiogenesis plays a critical role in the recovery and survival of patients and animal models of ischemic stroke. Panax notoginseng has been used as a key herb in the treatment of stroke diseases due to its effect in promoting blood circulation and removing blood stasis. However, the role of Panax notoginseng saponins, in promoting angiogenesis is unclear.
View Article and Find Full Text PDFEvolutionary Insights from Association Rule Mining of Co-Occurring Mutations in Influenza Hemagglutinin and Neuraminidase.
Viruses
September 2024
Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, Sydney, NSW 2145, Australia.
Seasonal influenza viruses continuously evolve via antigenic drift. This leads to recurring epidemics, globally significant mortality rates, and the need for annually updated vaccines. Co-occurring mutations in hemagglutinin (HA) and neuraminidase (NA) are suggested to have synergistic interactions where mutations can increase the chances of immune escape and viral fitness.
View Article and Find Full Text PDF[Mechanism of Xueshuantong Injection on bleomycin-induced pulmonary fibrosis in rats based on coagulation cascade pathway].
Zhongguo Zhong Yao Za Zhi
August 2024
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Article Synopsis
- This study explores how Xueshuantong Injection (XST) affects pulmonary fibrosis in rats induced by bleomycin (BLM), focusing on the coagulation pathway.
- Sixty rats were divided into different treatment groups, and after administering BLM to induce fibrosis, various treatments—including XST and a control drug—were applied to assess their impact on lung condition over 28 days.
- Results showed that XST treatment led to reduced collagen fibers in the lungs and lowered protein levels associated with fibrosis, while also normalizing coagulation markers and downregulating a key receptor involved in the fibrotic process.
-linked protein glycosylation in (formerly DPANN) archaea and their hosts.
J Bacteriol
September 2024
Super-cutting-edge Grand and Advanced Research (SUGAR) Program, Institute for Extra-cutting-edge Science and Technology Avant-garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
Members of the kingdom , previously known as DPANN archaea, are characterized by ultrasmall cell sizes and reduced genomes. They primarily thrive through ectosymbiotic interactions with specific hosts in diverse environments. Recent successful cultivations have emphasized the importance of adhesion to host cells for understanding the ecophysiology of .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!