AI Article Synopsis
- The study focuses on the EPIDERMAL PATTERNING FACTOR (EPF) and EPF-LIKE (EPFL) peptides, which are important for plant growth but not well-studied in rice (Oryza sativa).
- Using CRISPR/Cas9 technology, researchers knocked out 11 EPF/EPFL genes in a rice variety, finding that only the OsEPFL2 gene significantly affected traits like awn length and grain size.
- The research indicates that OsEPFL2 is crucial for young panicle development and has been positively selected during rice domestication, highlighting the diverse functional roles of gene family members.
Article Abstract
The EPIDERMAL PATTERNING FACTOR (EPF) and EPF-LIKE (EPFL) family of small secreted peptides act to regulate many aspects of plant growth and development; however, their functions are not widely characterized in rice (Oryza sativa). Here, we used clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) technology to individually knockout each of 11 EPF/EPFL genes in the rice cultivar Kasalath. Loss of function of most OsEPF/EPFL genes generated no obvious phenotype alteration, while disruption of OsEPFL2 in Kasalath caused a short or no awn phenotype and reduced grain size. OsEPFL2 is strongly expressed in the young panicle, consistent with a role in regulating awn and grain development. Haplotype analysis indicated that OsEPFL2 can be classified into six major haplotypes. Nucleotide diversity and genetic differentiation analyses suggested that OsEPFL2 was positively selected during the domestication of rice. Our work to systematically investigate the function of EPF/EPFL peptides demonstrates that different members of the same gene family have been independently selected for their ability to regulate a similar biological function and provides perspective on rice domestication.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434303 | PMC |
| http://dx.doi.org/10.1093/plphys/kiac278 | DOI Listing |
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