AI Article Synopsis
- A novel Electrochemiluminescence (ECL) biosensing platform was developed using Glutathione-Au nanoclusters on reduced graphene oxide (GSH-Au NCs@rGO) and copper oxide functionalized Au nanoparticles (Au@CuO) as a quenching probe.
- The GSH-Au NCs enhance ECL signal intensity by improving luminophore support and facilitating mass transfer, while Au@CuO enables effective resonance energy transfer to quench the signal for more accurate trace detection.
- This biosensing platform demonstrated a low detection limit of 54.95 fg/mL for cardiac troponin I (cTnI), showing potential for sensitive biomarker
Article Abstract
A "signal-off" Electrochemiluminescence (ECL) biosensing platform based on Glutathione-Au nanoclusters covered reduced graphene oxide (GSH-Au NCs@rGO) and Au nanoparticles functionalized copper oxide (Au@CuO) was fabricated. The GSH ligand protected Au NCs were spontaneously adsorbed on the rGO surface via Van der Waals force. As ECL emitters, GSH-Au NCs@rGO not only support more luminophores and immobilization of bioreceptor units also facilitates mass transfer, accelerating ECL excitation to obtain a higher ECL signal intensity. Remarkably, Au@CuO with good biocompatibility was first applied as a quenching probe. Au@CuO (acceptor)-dependent resonance energy transfers (RET) with GSH-Au NCs@rGO (donor) could effectively quenched the ECL intensity to a reasonable range for requirements of trace analysis. The proposed ECL biosensing platform was evaluated with cardiac troponin I (cTnI) as a model analyte, achieving a low detection limit of 54.95 fg/mL. This strategy may provide as new approaches for the sensitive detection of biomarkers in the early clinical diagnosis of diseases.
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| http://dx.doi.org/10.1016/j.talanta.2022.123649 | DOI Listing |
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