Plasma cfDNA for the Diagnosis and Prognosis of Colorectal Cancer.

J Oncol

Department of Gastrointestinal Surgery, The Seventh Affiliated Hospital of Yangzhou University, Jingjiang People's Hospital, Taizhou, Jiangsu, China.

Published: May 2022

Objective: To evaluate the value of cell-free DNA (cfDNA) for the diagnosis and prognosis of colorectal cancer (CRC).

Methods: Peripheral blood specimens of 120 CRC patients and 90 healthy volunteers (as a control cohort) were extracted. A quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine the cfDNA expression. Following correlation analyses for cfDNA and clinical endpoints, a receiver operator characteristic (ROC) curve was established to assess the sensitivity and specificity of cfDNA, CEA, VEGF, and CA125 and for evaluating the disease-free survival (DFS) of patients.

Results: The plasma cfDNA level of colorectal cancer patients was significantly higher than that of healthy subjects ( < 0.05), and after chemotherapy, cfDNA level was significantly lower than that before chemotherapy ( < 0.05). CA125/CEA/VEGF expression significantly correlated with cfDNA level, but not with cfDNA integrity. There was also a significant correlation between tumor differentiation and the cfDNA level. cfDNA has a higher ROC value than the current tumor biomarkers. Survival analysis showed that the DFS of the low cfDNA expression group was longer (29.99 ± 0.78 months) than that of the high cfDNA expression group (27.66 ± 1.05 months, =0.031).

Conclusion: The blood cfDNA is associated with the pathological features of CRC clinical cases and represents a possible indicator for CRC diagnosis and prognosis.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174014PMC
http://dx.doi.org/10.1155/2022/9538384DOI Listing

Publication Analysis

Top Keywords

cfdna level
16
cfdna
13
diagnosis prognosis
12
colorectal cancer
12
cfdna expression
12
plasma cfdna
8
cfdna diagnosis
8
prognosis colorectal
8
level cfdna
8
expression group
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!