Recent backscattering interferometry studies utilise a single channel microfluidic system, typically approximately semicircular in cross-section. Here, we present a complete ray tracing model for on-chip backscattering interferometry with a semicircular cross-section, including the dependence upon polarisation and angle of incidence. The full model is validated and utilised to calculate the expected fringe patterns and sensitivities observed under both normal and oblique angles of incidence. Comparison with experimental data from approximately semicircular channels using the parameters stated shows that they cannot be explained using a semicircular geometry. The disagreement does not impact on the validity of the experimental data, but highlights that the optical mechanisms behind the various modalities of backscattering interferometry would benefit from clarification. From the analysis presented here, we conclude that for reasons of ease of analysis, data quality, and sensitivity for a given radius, capillary-based backscattering interferometry affords numerous benefits over on-chip backscattering interferometry.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185450 | PMC |
| http://dx.doi.org/10.3390/s22114301 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microscale quantitation of heavy metals by back-scattering interferometry in conjunction with photothermal effect using a single laser beam.
Talanta
December 2024
Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada. Electronic address:
A microanalytical technique based on the photothermal effect in conjunction with back-scattering interferometry (BSI) using a single laser beam was developed for quantitative detection of heavy metals. After the chromogenic reaction of an analyte in a capillary tube, the photothermal effect induced by irradiation with the same laser beam leads to a change of the refractive index of the solution, which can be "quantified" using the BSI technique. For prove-of-concept, Cu(II) was chosen as the trial analyte, for which the solution changes to purplish through reacting with the chromogenic reagent; a single laser beam of 532 nm was adapted for both inducing the photothermal effect and realizing BSI detection.
View Article and Find Full Text PDFPoint-cloud clustering and tracking algorithm for radar interferometry.
Phys Rev E
October 2024
Department of Chemistry and Physics, Mount Royal University, Calgary, Alberta, Canada.
In data mining, density-based clustering, which entails classifying datapoints according to their distributions in some space, is an essential method to extract information from large datasets. With the advent of software-based radio, ionospheric radars are capable of producing unprecedentedly large datasets of plasma turbulence backscatter observations, and new automatic techniques are needed to sift through them. We present an algorithm to automatically identify and track clusters of radar echoes through time, using dbscan, a celebrated density-based clustering method for noisy point clouds.
View Article and Find Full Text PDFPhotothermal Backscatter Interferometry for Enhanced Detection in Capillary Electrophoresis.
Anal Chem
August 2024
Ralph N. Adams Institute for Bioanalytical Chemistry, Department of Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, Kansas 66047, United States.
Refractive index (RI) detection using backscatter interferometry (BSI) enables universal detection in capillary electrophoresis (CE). BSI detection is a versatile on-capillary approach that is easily integrated with capillary or microfluidic channels, straightforward to miniaturize, and inexpensive. The focused BSI light source can also double as the excitation source for fluorescence, enabling simultaneous universal (BSI) and specific (fluorescence) signals from the same detection volume.
View Article and Find Full Text PDFBichromatic tetraphasic full-field optical coherence microscopy.
J Biomed Opt
June 2024
University of Illinois Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Urbana, Illinois, United States.
Significance: Full-field optical coherence microscopy (FF-OCM) is a prevalent technique for backscattering and phase imaging with epi-detection. Traditional methods have two limitations: suboptimal utilization of functional information about the sample and complicated optical design with several moving parts for phase contrast.
Aim: We report an OCM setup capable of generating dynamic intensity, phase, and pseudo-spectroscopic contrast with single-shot full-field video-rate imaging called bichromatic tetraphasic (BiTe) full-field OCM with no moving parts.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!