AI Article Synopsis
- Previous research using untargeted LC-MS analysis identified a range of significant compounds varying across different ssp. cultivars, lacking reference compounds for dihydro-isocoumarins.
- Researchers focused on isolating and characterizing these compounds from the 'Yae-no-amacha' cultivar through NMR and LC-MS techniques.
- Thirteen dihydro-isocoumarins, along with other metabolites, were successfully isolated and identified, including new dihydrostilbenoid glycosides, providing valuable insights for future biological studies.
Article Abstract
Previously, different ssp. cultivars were investigated by untargeted LC-MS analysis. From this, a list of tentatively identified and unknown compounds that differ significantly between these cultivars was obtained. Due to the lack of reference compounds, especially for dihydro-isocoumarins, we aimed to isolate and structurally characterise these compounds from the cultivar 'Yae-no-amacha' using NMR and LC-MS methods. For purification and isolation, counter-current chromatography was used in combination with reversed-phase preparative HPLC as an orthogonal and enhanced purification workflow. Thirteen dihydro-isocoumarins in combination with other metabolites could be isolated and structurally identified. Particularly interesting was the clarification of dihydrostilbenoid glycosides, which were described for the first time in ssp. . These results will help us in further studies on the biological interpretation of our data.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182509 | PMC |
| http://dx.doi.org/10.3390/molecules27113424 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!