(L.) L'Hér. ex Vent. has considerable economic and ecological value and a long history of use in China. In this paper, root cuttings were used as the material to establish an efficient vegetative propagation of . The results revealed that root segments with a diameter of 1.5~2.0 cm and a length of 20~30 cm were most suitable for shoot regeneration, as these segments had the highest adventitious shoot induction rates (93.3%), strongest adventitious shoots, and highest multiplication coefficients (7.07). With regard to the methods used for root burial, a horizontal burial at a depth of 1~3 cm yielded the best results, in this case, the adventitious shoot induction rate can reach 86.7%. The best substrate combination was perlite: peat: coconut chaff = 1:1:1 (//), wherein the adventitious shoot induction rate can reach 75.6%. The best sterilization method was mixing soil with carbendazim and soaking the root sections in carbendazim for 30 min, wherein the adventitious shoot induction rate can reach 77.8%. Adding 0.2 mg/L naphthaleneacetic acid (NAA) to 1/4 Hoagland nutrient solution significantly improved the rooting rate of adventitious shoots to 82.2%, and the survival rate of the acclimatized plants was more than 90.0%.
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http://dx.doi.org/10.3390/plants11111423 | DOI Listing |
Physiol Plant
December 2024
Graduate School of Life Sciences, Toyo University, Asaka-shi, Saitama, Japan.
In many plant species, the application of exogenous phytohormones is crucial for initiating de novo shoot regeneration. However, ipecac [Carapichea ipecacuanha (Brot) L. Andersson] has a unique ability to develop adventitious shoots on the epidermis of internodal segments without phytohormone treatment.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Background: Embryogenic callus (EC) has strong regenerative potential, useful for propagation and genetic transformation. miRNAs have been confirmed to play key regulatory roles in EC regeneration across various plants. However, challenges in EC induction have hindered the breeding of drumstick (Moringa oleifera Lam.
View Article and Find Full Text PDFPlants (Basel)
December 2024
College of Horticulture, Hebei Agricultural University, Baoding 071000, China.
Organic acids are naturally present in plants and exert a positive influence on plant development, which justifies surveying their potential effect on adventitious root (AR) formation. In this study, 0.0298 mol/L (4000 mg/L) of malic acid and 0.
View Article and Find Full Text PDFPlants (Basel)
November 2024
Germplasm Conservation Laboratory, Department of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Unconventional germination, wherein shoots emerge and establish true leaves before the root emerges, is only found in Zosteraceae. In , germination proceeds with the primary root emerging, followed by shoot emergence on the opposite side, but before leaf differentiation, adventitious roots emerge at the base of the shoots. However, germination and survival mechanisms in several species are still unclear.
View Article and Find Full Text PDFFront Microbiol
November 2024
College of Horticulture and Gardening, Yangtze University, Jingzhou, China.
Introduction: Temporary and extended drought stress accelerates phytohormones and reactive oxygen species (ROS) in plants, however, the fate of the plants under stress is mostly determined by the metabolic and molecular reprogramming, which can be modulated by the application of habitat-adapted fungi that triggers resistance to stress upon symbiotic association.
Methods: The present research exhibited the exploitation of the newly isolated, drought habitat-adapted fungal endophytic consortium of SAB () and CBW (), on maize under drought stress. SAB and CBW primarily hosted the root tissues of L.
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