Elucidating the cellular and molecular mechanisms that regulate the balance between progenitor cell proliferation and neuronal differentiation in the construction of the embryonic brain demands the combination of cell lineage and functional approaches. Here, we generate the comprehensive lineage of hindbrain boundary cells by using a CRISPR-based knockin zebrafish transgenic line that specifically labels the boundaries. We unveil that boundary cells asynchronously engage in neurogenesis undergoing a functional transition from neuroepithelial progenitors to radial glia cells, coinciding with the onset of Notch3 signaling that triggers their asymmetrical cell division. Upon notch3 loss of function, boundary cells lose radial glia properties and symmetrically divide undergoing neuronal differentiation. Finally, we show that the fate of boundary cells is to become neurons, the subtype of which relies on their axial position, suggesting that boundary cells contribute to refine the number and proportion of the distinct neuronal populations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.celrep.2022.110915 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intercellular communication is crucial in the regulation of healthy aging via exosomes.
Pharmacol Res
January 2025
College of Biological and Food Engineering, Qujing Normal University, 655011, Qujing, Yunnan, China. Electronic address:
The hallmarks of aging encompass a variety of molecular categories (genomic, telomeric, and epigenetic), organelles (proteostasis, autophagy, and mitochondria), cellular components (including stem cells), systems (such as intercellular communication and chronic inflammation), and environmental factors (dysbiosis and nutrient sensing). These hallmarks play a crucial role in the aging process. Despite their intricate interconnections, the relationships among the hallmarks of aging remain unclear.
View Article and Find Full Text PDFModeling of Electric Field and Dielectrophoretic Force in a Parallel-Plate Cell Separation Device with an Electrode Lid and Analytical Formulation Using Fourier Series.
Sensors (Basel)
December 2024
Department of Applied Physics, National Defense Academy, Hashirimizu 1-10-20, Yokosuka 239-0802, Kanagawa, Japan.
Dielectrophoresis (DEP) cell separation technology is an effective means of separating target cells which are only marginally present in a wide variety of cells. To develop highly efficient cell separation devices, detailed analysis of the nonuniform electric field's intensity distribution within the device is needed, as it affects separation performance. Here we analytically expressed the distributions of the electric field and DEP force in a parallel-plate cell separation DEP device by employing electrostatic analysis through the Fourier series method.
View Article and Find Full Text PDFMultifunctional Organic Molecule for Defect Passivation of Perovskite for High-Performance Indoor Solar Cells.
Materials (Basel)
January 2025
Key Laboratory of Applied Surface and Colloid Chemistry, National Ministry of Education, Shaanxi Key Laboratory for Advanced Energy Devices, Shaanxi Engineering Laboratory for Advanced Energy Technology, School of Materials Science and Engineering, Shaanxi Normal University, Xi'an 710119, China.
Perovskite solar cells (PSCs) can utilize the residual photons from indoor light and continuously supplement the energy supply for low-power electron devices, thereby showing the great potential for sustainable energy ecosystems. However, the solution-processed perovskites suffer from serious defect stacking within crystal lattices, compromising the low-light efficiency and operational stability. In this study, we designed a multifunctional organometallic salt named sodium sulfanilate (4-ABS), containing both electron-donating amine and sulfonic acid groups to effectively passivate the positively-charged defects, like under-coordinated Pb ions and iodine vacancies.
View Article and Find Full Text PDFRetinal ganglion cells encode the direction of motion outside their classical receptive field.
Proc Natl Acad Sci U S A
January 2025
Department of Brain Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel.
Retinal ganglion cells (RGCs) typically respond to light stimulation over their spatially restricted receptive field. Using large-scale recordings in the mouse retina, we show that a subset of non- direction-selective (DS) RGCs exhibit asymmetric activity, selective to motion direction, in response to a stimulus crossing an area far beyond the classic receptive field. The extraclassical response arises via inputs from an asymmetric distal zone and is enhanced by desensitization mechanisms and an inherent DS component, creating a network of neurons responding to motion toward the optic disc.
View Article and Find Full Text PDFNematic liquid crystal flow driven by time-varying active surface anchoring.
Soft Matter
January 2025
Faculty of Mathematics and Physics, University of Ljubljana, Jadranska 19, 1000 Ljubljana, Slovenia.
We demonstrate the generation of diverse material flow regimes in nematic liquid cells as driven by time-variable active surface anchoring, including no-net flow, oscillatory flow, steady flow, and pulsating flow. Specifically, we numerically simulate a passive nematic fluid inside a cell bounded with two flat solid boundaries at which the time-dependent anchoring is applied with the dynamically variable surface anchoring easy axis. We show that different flow regimes emerge as the result of different anchoring driving directions ( co-rotating or counter-rotating) and relative phase of anchoring driving.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!