The Notch pathway regulates many cellular functions in a context-dependent manner. Depending on the cell type, either the activation or inhibition of Notch signaling can influence many processes such as cellular proliferation, specification, differentiation, and survival. The activation of Notch signaling has been shown to have therapeutic advantages in some cancers, thus having a method to identify Notch-activating compounds is needed. In this chapter we outline a method for high-throughput analysis of potential Notch pathway activators in a pancreatic neuroendocrine tumor cell line as an example. We also include the steps for subsequent validation of results and preclinical testing.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-0716-2201-8_5DOI Listing

Publication Analysis

Top Keywords

notch signaling
12
high-throughput analysis
8
notch pathway
8
notch
5
analysis identify
4
identify activators
4
activators notch
4
signaling notch
4
pathway regulates
4
regulates cellular
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!