AI Article Synopsis
- The study establishes long-term cultures of both murine and human salivary gland organoids that retain gland-specific genes and functions, helping to better understand salivary gland biology.
- Human organoids can be derived from different cell types while maintaining their characteristics, indicating the ability to replicate the diversity of human salivary glands.
- The research also enables the creation of tumoroid cultures for various types of salivary gland tumors, providing a platform for advancements in precision medicine and cancer treatment.
Article Abstract
Salivary glands that produce and secrete saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. The long-term maintenance of diverse salivary gland cells in organoids remains problematic. Here, we establish long-term murine and human salivary gland organoid cultures. Murine and human salivary gland organoids express gland-specific genes and proteins of acinar, myoepithelial, and duct cells, and exhibit gland functions when stimulated with neurotransmitters. Furthermore, human salivary gland organoids are established from isolated basal or luminal cells, retaining their characteristics. Single-cell RNA sequencing also indicates that human salivary gland organoids contain heterogeneous cell types and replicate glandular diversity. Our protocol also enables the generation of tumoroid cultures from benign and malignant salivary gland tumor types, in which tumor-specific gene signatures are well-conserved. In this study, we provide an experimental platform for the exploration of precision medicine in the era of tissue regeneration and anticancer treatment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174290 | PMC |
| http://dx.doi.org/10.1038/s41467-022-30934-z | DOI Listing |
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