Studies on the activated sludge process crucial parameters controlling olive mill wastewater treatment.

Olive mill wastewater (OMW) represents a severe environmental problem for Mediterranean producer countries. The release of OMW, in urban areas, in public sewerage system constitute a serious problem for wastewater treatment plant (WWTP) based on biological processes. This study delivers a critical and a comprehensive analysis on activated sludge biomass and process crucial parameters involved in the treatment of OMW. Principal component analysis (PCA) has been applied to master the relationship between activated sludge physiological state and process performances stability. After the acclimation step with easily biodegradable synthetic substrate (SS), increased OMW F/M mass ratio has been applied to the bioreactor (60 L) of experimental pilot-scale AS system in respect to its nominal capacity. PCA highlight two main periods, a first stressful period presented by G[T-T], G[T-T] and G[T-T] AS microbial communities were very sensitive to substrate changes. This was manifested by low metabolic activity (OUR, SOUR and SOUR), important drop in AS microbial communities content, an increase in soluble microbial products (SMP), a fluctuate performances in COD and total phenols abatements as well as in flocks settleability (SVI). Afterwards, a reestablishment second period presented by G[T-T] of key process parameters and AS biomass efficiency indices is reached. This period characterized by the substantial content, resistance and adaption of AS microbial communities to OMW as F/M. Succeeding optimal AS biomass and process performances, under stressful conditions, requires a precise knowledge and control of decisive parameters involved in OMW treatment and this study could be an efficient practical approach in case of OMW also for further toxic compounds.