Background: Myricetin (Myr) is a flavonoid compound that exist widely in many natural plants. Myr has been proven to have multiple biological functions, including immunomodulatory and anti-inflammatory effects.
Purpose: In this study, we investigated the therapeutic effect of Myr on calcipotriol (MC903) induced atopic dermatitis (AD) mouse model and tumor necrosis factor (TNF)-α/interferon (IFN)-γ stimulated human immortal keratinocyte line (HaCaT) in vivo and in vitro.
Methods: MC903 was applied topically to the left ears of mice to establish AD mouse model. After the AD model established successfully, the cream base, dexamethasone (DEX) cream or Myr cream were applied on the lesions of mice for 8 days. Through measuring ear thickness and scoring dermatitis severity, we evaluated the therapeutic effect of Myr, the draining lymph nodes (DLNs) and ears of the mice were collected for mechanistic study. In addition, TNF-α and IFN-γ-activated HaCaT cells were used to investigate the underlying mechanism.
Results: Our data demonstrated that Myr alleviated the symptoms of AD by exerting anti-inflammatory and anti-allergic functions in vivo. We found that Myr treatment suppressed ear swelling and IgE level in the serum, reduced the infiltration of mast cells in skin lesions, decreased expressions of thymus and activation regulated chemokine (TARC), IL-4, IFN-γ and thymic stromal lymphopoietin (TSLP) in ear lesions, increased the expressions of filaggrin (FLG). Furthermore, our experimental results demonstrated that Myr down-regulated the mRNA expressions of T-bet and GATA-3 in DLNs. In vitro, Myr treatment decreased MDC and TARC expressions in IFN-γ and TNF-α-induced HaCaT cells by blocking the NF-κB and STAT1 signal pathway.
Conclusion: The present study is the first to investigate the anti-atopic effects of Myr. Our findings suggested the therapeutic effects of Myr against MC903-induced AD-like skin lesions in mice. Therefore, Myr may be a potential therapeutic agent for AD.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.phymed.2022.154200 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The initial solar system abundance of Fe and early core formation of the first asteroids.
Sci Adv
January 2025
Université de Lorraine, CNRS, CRPG, UMR7358, F-54000 Nancy, France.
High-precision Ni isotope analyses of the differentiated andesitic meteorite Erg Chech 002 (EC 002), the oldest known crustal fragment of a planetesimal, show that short-lived Fe was present in the early solar system with an initial Fe/Fe ratio of (7.71 ± 0.47) × 10, which is five times more precise than previous estimates and is proposed to be the reference value for further studies.
View Article and Find Full Text PDFInteractions between magma oceans and overlying atmospheres on young rocky planets leads to an evolving feedback of outgassing, greenhouse forcing, and mantle melt fraction. Previous studies have predominantly focused on the solidification of oxidized Earth-similar planets, but the diversity in mean density and irradiation observed in the low-mass exoplanet census motivate exploration of strongly varying geochemical scenarios. We aim to explore how variable redox properties alter the duration of magma ocean solidification, the equilibrium thermodynamic state, melt fraction of the mantle, and atmospheric composition.
View Article and Find Full Text PDFDe Novo Design of Lipopeptide-Based β-Sheet-Like Self-Assemblies: A Strategy to Develop Fusion Inhibitors as Broad-Spectrum Antivirals.
Chemistry
December 2024
"Indian Institute of Science Education and Research Kolkata", Chemical Sciences, Research Complex, 741246, Mohanpur, INDIA.
The recent surge in emerging viral infections warrants the design of broad-spectrum antivirals. We aim to develop a lead molecule that targets a common biochemical feature of many enveloped viruses, membrane fusion. To achieve the broad-spectrum ability, instead of targeting the fusion machinery, we plan to modulate the physicochemical properties of the host and viral membranes to block fusion.
View Article and Find Full Text PDFEarly-middle Permian Mediterranean gorgonopsian suggests an equatorial origin of therapsids.
Nat Commun
December 2024
Institut Català de Paleontologia Miquel Crusafont (ICP-CERCA), Universitat Autònoma de Barcelona, Edifici ICTA-ICP, c/ Columnes s/n, Campus de la UAB, 08193, Cerdanyola del Vallès, Barcelona, Catalunya, Spain.
Article Synopsis
- Therapsids were key players in ancient ecosystems and evolved into mammals during the early Mesozoic, but their origins are not well understood.
- A newly discovered partial skeleton of a gorgonopsian from Mallorca is possibly the oldest known therapsid, dating back to the lower-middle Permian period.
- Using advanced dating methods, researchers created a timeline showing that therapsids diversified rapidly after Olson's Extinction, suggesting they emerged in tropical Pangaea's wet biome rather than in cooler regions.
Optimization of sulforaphane bioavailability from a glucoraphanin-rich broccoli seed extract in a model of dynamic gastric digestion and absorption by Caco-2 cell monolayers.
Food Funct
January 2025
Nutrition and Environmental Toxicology, University of California Davis, Davis, CA, USA.
Broccoli is recognized for its health benefits, attributed to the high concentrations of glucoraphanin (GR). GR must be hydrolyzed by myrosinase (Myr) to form the bioactive sulforaphane (SF). The primary challenge in delivering SF in the upper gastrointestinal (GI) tract- is improving hydrolysis of GR to SF.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!