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Binding of the transcription activator-like effector augments transcriptional regulation by another transcription factor. | LitMetric

AI Article Synopsis

  • DNA transcription is primarily regulated by transcription factors that help RNA polymerase bind to DNA, and proteins can influence this process by interacting with these transcription factors.
  • Research found that a specific type of protein called a Transcription Activator-Like Effector (TALE) can enhance transcription in mammalian cells without directly interacting with transcription factors, working effectively across different recognition sequences and orientations.
  • The study suggests that TALEs might function by creating an allosteric effect on DNA structure, which could be useful for manipulating transcription and understanding their natural role.

Article Abstract

DNA transcription is regulated by a range of diverse mechanisms and primarily by transcription factors that recruit the RNA polymerase complex to the promoter region on the DNA. Protein binding to DNA at nearby or distant sites can synergistically affect this process in a variety of ways, but mainly through direct interactions between DNA-binding proteins. Here we show that a Transcription Activator-Like Effector (TALE), which lacks an activation domain, can enhance transcription in mammalian cells when it binds in the vicinity of and without direct interaction with several different dimeric or monomeric transcription factors. This effect was observed for several TALEs regardless of the recognition sequences and their DNA-bound orientation. TALEs can exert an effect over the distance of tens of nucleotides and it also potentiated KRAB-mediated repression. The augmentation of transcriptional regulation of another transcription factor is characteristic of TALEs, as it was not observed for dCas9/gRNA, zinc finger, or Gal4 DNA-binding domains. We propose that this mechanism involves an allosteric effect exerted on DNA structure or dynamics. This mechanism could be used to modulate transcription but may also play a role in the natural context of TALEs.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226504PMC
http://dx.doi.org/10.1093/nar/gkac454DOI Listing

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