AI Article Synopsis
- Endophytes can enhance plant growth and stress resistance by producing bioactive compounds like thiamine.
- In a study, thiamine was found to inhibit the rice blast pathogen and improve resistance under real-world conditions.
- The endophyte OsiSh-2 and rice have similar thiamine-synthesizing enzymes, and their interaction can regulate thiamine levels to boost rice’s resistance to disease.
Article Abstract
Endophytes can benefit the growth and stress resistance of host plants by secreting bioactive components. Thiamine is an essential vitamin involved in many metabolic pathways and can only be synthesized by microbes and plants. In this study, we found that thiamine could inhibit the development of the phytopathogen and decrease the rice blast index under field conditions. In the thiamine biosynthesis pathway, the key enzyme ShTHIC of an endophyte OsiSh-2 and OsTHIC of rice () were highly homologous. Gene overexpression or knockout approaches revealed that both THIC contributed to thiamine synthesis and resistance to . Furthermore, OsiSh-2 colonization led to a decrease in the thiamine synthesis level of rice but still maintained thiamine homeostasis in rice. However, inoculation with the ShTHIC knockout strain Δ reduced the thiamine content in rice, although the thiamine synthesis level of rice was increased. After infection with , blast resistance was dramatically improved in OsiSh-2-inoculated rice but decreased in Δ-inoculated rice compared with non-inoculated rice. This result demonstrated that could regulate thiamine biosynthesis and consequently assist blast resistance in the OsiSh-2-rice symbiont. Our results revealed a novel blast-resistance mechanism mediated by a key thiamine biosynthetic enzyme from an endophyte OsiSh-2.
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| http://dx.doi.org/10.1021/acs.jafc.2c00776 | DOI Listing |
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