Batch Experiments Demonstrating a Two-Stage Bacterial Process Coupling Methanotrophic and Heterotrophic Bacteria for 1-Alkene Production From Methane.

Methane (CH) is a sustainable carbon feedstock for value-added chemical production in aerobic CH-oxidizing bacteria (methanotrophs). Under substrate-limited (e.g., oxygen and nitrogen) conditions, CH oxidation results in the production of various short-chain organic acids and platform chemicals. These CH-derived products could be broadened by utilizing them as feedstocks for heterotrophic bacteria. As a proof of concept, a two-stage system for CH abatement and 1-alkene production was developed in this study. Type I and Type II methanotrophs, SV96 and SV97, respectively, were investigated in batch tests under different CH and air supplementation schemes. CH oxidation under either microaerobic or aerobic conditions induced the production of formate, acetate, succinate, and malate in SV96, accounting for 4.8-7.0% of consumed carbon from CH (C-CH), while SV97 produced the same compounds except for malate, and with lower efficiency than SV96, accounting for 0.7-1.8% of consumed C-CH. For the first time, this study demonstrated the use of organic acid-rich spent media of methanotrophs cultivating engineered ADP1 ' cells for 1-alkene production. The highest yield of 1-undecene was obtained from the spent medium of SV96 at 68.9 ± 11.6 μmol mol C . However, further large-scale studies on fermenters and their optimization are required to increase the production yields of organic acids in methanotrophs.