The existence of cyanotoxins poses serious threats to human health, it is highly desirable to develop specific and sensitive methods for rapid detection of cyanotoxins in food and water. Due to the distinct advantages of aptamer including high specificity, good stability and easy preparation, various aptamer-based sensors (aptasensors) have been proposed to promote the detection of cyanotoxins. In this review, we summarize recent advance in optical and electrochemical aptasensors for cyanotoxins sensing by integrating with versatile nanomaterials or innovative sensing strategies, such as colorimetric aptasensors, fluorescent aptasensors, surface enhancement Raman spectroscopy-based aptasensors, voltammetric aptasensors, electrochemical impedance spectroscopy-based aptasensors and photoelectrochemical aptasensors. We highlight the accomplishments and advancements of aptasensors with improved performance. Furthermore, the current challenges and future prospects in cyanotoxins detection are discussed from our perspectives, which we hope to provide more ideas for future researchers.
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http://dx.doi.org/10.1016/j.talanta.2022.123607 | DOI Listing |
Toxins (Basel)
January 2025
Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.
Grazing by zooplankton can regulate bloom-forming cyanobacteria but can also transfer toxin-producing cells, as well as toxic metabolites, to the food web. While laboratory investigations have provided extensive knowledge on zooplankton and toxic cyanobacteria interactions, information on zooplankton feeding on toxin-producing cyanobacteria in natural water bodies remains scarce. In this study, we quantified -specific synthase genes from the gut contents of various cladoceran and copepod taxa to assess the in situ crustacean community and taxon-specific ingestion of potentially toxic in Lake Peipsi, a large eutrophic lake in Estonia, Northern Europe.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Donghu Experimental Station of Lake Ecosystems, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, PR China; Southwest United Graduate School, Kunming 650092, PR China. Electronic address:
Cyanobacteria blooms are concerning due to algal toxins like microcystin-leucine arginine (MC-LR). Despite progress in detecting MC-LR and understanding its toxic effects, including calf thymus DNA (CT-DNA) damage, the mechanisms for fluorescent probe detection of MC-LR and its binding to CT-DNA are poorly understood. In this study, we designed three fluorescent probes for MC-LR detection.
View Article and Find Full Text PDFJ Toxicol Environ Health A
January 2025
School of Public Health, Hengyang Medical School, University of South China, Hengyang, China.
The global phenomenon of cyanobacterial bloom pollution is spreading globally due to climate change and eutrophication. It is well established that harmful cyanobacteria produce a wide range of toxins including microcystin-LR (MC-LR), a cyclic heptapeptide toxin known to damage various organs. The intestinal tract is the main site of MC-LR absorption and one of the targets susceptible to toxicity.
View Article and Find Full Text PDFAnal Chem
January 2025
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
The rapid, sensitive, and accurate detection of paralytic shellfish toxins (PSTs), such as saxitoxin (STX), is critical for protecting human health due to the frequent occurrence of toxic red tides. In this work, to address the low affinity of traditional mouse monoclonal antibodies (m-mAbs), rabbit monoclonal antibodies (r-mAbs) against STX were produced by a single B-cell sorting culture and a cross-selection strategy. The r-mAbs showed 100-fold improvement in sensitivity (IC = 0.
View Article and Find Full Text PDFPathogens
November 2024
New Brunswick Research and Productivity Council (RPC), 921 College Hill Rd, Fredericton, NB E3B 6Z9, Canada.
Harmful cyanobacterial blooms produce cyanotoxins which can adversely affect humans and animals. Without proper monitoring and detection programs, tragedies such as the loss of pets or worse are possible. Multiple factors including rising temperatures and human influence contribute to the increased likelihood of harmful cyanobacteria blooms.
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