The quality evaluation of nature polysaccharides is a tough nut to crack because of its high M distributions and larger polarity property. It is well-known that infrared spectroscopy and multiple regression modeling have been used for quantitative examinations in multiple fields, but it has not been applied to the compositional analysis of polysaccharides. In this study, attenuated total reflectance-fourier transform infrared spectroscopy is used to simultaneously quantify aldoses, ketose and uronic acids in Atractylodes polysaccharides by a combination of multivariate regressions. After experience of different data processing pretreatments, the resulting spectrum contains maximum amount of information of monosaccharide contents in Atractylodes polysaccharides. In this case, different smoothing points, derivatives, SNV and MSC are used in the pre-modeling spectrum processing and VIP screening is used to reduce the number of variables to simplify the calculation of the model. All the most optimal prediction models have both good prediction ability (R ≥ 0.9 and RPD > 3) and no over fitting (RMSEP/RMSEC < 3). This strategy has opened a new possibility for the nondestructive determination of complex monosaccharide compositions of natural polysaccharides in a short detection time, low equipment requirement and high experimental safety.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.saa.2022.121411 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Polysaccharides of Atractylodes Macrocephala Koidz Alleviate LPS-Induced Bursa of Fabricius Injury in Goslings by Inhibiting EREG Expression.
Animals (Basel)
January 2025
College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
The bursa of Fabricius (BF) plays crucial roles in the goslings' immune system. During waterfowl breeding, the presence of lipopolysaccharides (LPSs) in the environment can induce inflammatory damage in geese. Polysaccharides of Atractylodes macrocephala Koidz (PAMKs), as the main active component of the Chinese medicine Atractylodes macrocephala, have significant immune-enhancing effects.
View Article and Find Full Text PDFAtractylodes macrocephala Koidz polysaccharide ameliorates DSS-induced colitis in mice by regulating the gut microbiota and tryptophan metabolism.
Br J Pharmacol
December 2024
State Key Laboratory of Natural Medicines, New Drug Screening and Pharmacodynamics Evaluation Center, China Pharmaceutical University, Nanjing, China.
Background And Purpose: Ulcerative colitis (UC) is an idiopathic inflammatory bowel disease, and the range of current clinical treatments is not ideal. We previously found that polysaccharide of Atractylodes macrocephala Koidz (PAMK) is beneficial in DSS-induced colitis, and we aimed to investigate the underlying mechanisms in this study.
Experimental Approach: PAMK was used to treat DSS-induced colitis in mice, 16S rRNA sequencing analysis was used to detect changes in the intestinal microbiota, targeted metabolomics analysis was used to determine the content of tryptophan-metabolizing bacteria, and western blotting was used to determine aryl hydrocarbon receptor (AhR) and pregnane X receptor (PXR) levels.
Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens.
Poult Sci
January 2025
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China. Electronic address:
This study aimed to investigate the effects of the in-ovo injection of Yu Ping Feng polysaccharides (YPF) on the immunological development, hatchability, growth performance, intestinal tissue development, intestinal IgA cell distribution, and intestinal secretory IgA (SIgA) production in broiler chicken. Herein, 800 chicken embryos were randomly divided into Astragalus polysaccharide (APS), Atractylodes macrocephala polysaccharide (ATR), YPF, and normal saline (control) injection groups-polysaccharide injection, 0.5 mL of 4 mg/mL polysaccharide; normal saline injection, 0.
View Article and Find Full Text PDFPAMK Ameliorates Non-Alcoholic Steatohepatitis and Associated Anxiety/Depression-like Behaviors Through Restoring Gut Microbiota and Metabolites in Mice.
Nutrients
November 2024
School of Nursing, Jinan University, Guangzhou 510632, China.
Objectives: Long-term Western diet-induced non-alcoholic steatohepatitis (NASH) can lead to liver cirrhosis and NASH-associated hepatocellular carcinoma, which are end-stage liver diseases. Meanwhile, NASH is associated with mental burden and worsens as the disease progresses. Atractylodes Macrocephala Koidz (AMK) is one of the main ingredients of Shenling Baizhu San, and the effect of Polysaccharide from AMK ameliorates (PAMK), as an important medicinal ingredient of AMK, on NASH and associated anxiety/depression-like behaviors is still unclear.
View Article and Find Full Text PDFResearch progress on extraction, separation, structure, and biological activities of polysaccharides from the genus Atractylodes: A review.
Int J Biol Macromol
December 2024
Wuya College of Innovation, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address:
Plants of the genus Atractylodes are perennial herbaceous plants in the family Asteraceae, whose rhizome is often used in the production of medicines and health products. There are 6 main species in this genus, namely A. macrocephala, A.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!